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使用抑制酶联免疫吸附测定法对疫苗中的荚膜多糖或蛋白质进行定量。

Use of an inhibition enzyme-linked immunosorbent assay for quantification of capsular polysaccharide or proteins in vaccines.

作者信息

Inzana Thomas J, Champion Anna

机构信息

Center for Molecular Medicine and Infectious Diseases, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA.

出版信息

Clin Vaccine Immunol. 2007 Mar;14(3):323-7. doi: 10.1128/CVI.00302-06. Epub 2007 Jan 31.

Abstract

An inhibition enzyme-linked immunosorbent assay (ELISA) is described for quantification of capsular polysaccharide or proteins in vaccines and other samples containing whole cells or extracts of Actinobacillus pleuropneumoniae. The assay can be used to quantify any antigen that can be purified and for which highly specific antibodies are not available. The assay can be carried out by any laboratory capable of performing an ELISA.

摘要

描述了一种抑制酶联免疫吸附测定(ELISA),用于定量疫苗以及含有胸膜肺炎放线杆菌全细胞或提取物的其他样品中的荚膜多糖或蛋白质。该测定可用于定量任何可纯化且没有高特异性抗体的抗原。任何能够进行ELISA的实验室均可开展此测定。

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