Lee Jae-Jin, Srinivasan Sathiyaraj, Lim Sangyong, Joe Minho, Im Seonghun, Kim Myung Kyum
Department of Bio and Environmental Technology, College of Natural Science, Seoul Women's University, Seoul, 139-774, Korea.
Curr Microbiol. 2015 Apr;70(4):464-9. doi: 10.1007/s00284-014-0748-8. Epub 2014 Dec 5.
A Gram-positive, coccus-shaped, crimson-color-pigmented bacterium was isolated from soil irradiated with 5 kGy gamma radiation and was designated strain DY1(T). Cells showed growth at 10-30 °C and pH 7-11 and were oxidase-negative and catalase-positive. Phylogenetic analyses of the 16S rRNA gene showed that the strain DY1(T) belonged to the genus Deinococcus with sequence similarities to Deinococcus aquatilis CCUG 53370(T) (96.2 %) and Deinococcus navajonensis KR-114(T) (94.1 %). Strain DY1(T) showed low level of DNA relatedness with D. aquatilis CCUG 53370(T) (41.3 ± 3.9 %). The DNA G + C content of DY1(T) was 58.7 mol%. Predominant fatty acids were summed feature 3 (C16:1 ω7c/ω6c), C16:0, and C17:0. The major amino acids were D-alanine, L-glutamic acid, glycine, and L-ornithine in the peptidoglycan. The major polar lipids were unknown phosphoglycolipids (PGL). Strain DY1(T) has resistance to gamma radiation and was found to be a novel species. Therefore, the strain was designated as DY1(T) (=KCTC 33027(T) = JCM 18576(T)), and the name Deinococcus puniceus sp. nov. is herein proposed.
从经5千戈瑞伽马辐射照射的土壤中分离出一株革兰氏阳性、球菌状、呈深红色色素的细菌，命名为菌株DY1(T)。该菌在10 - 30°C和pH 7 - 11条件下生长，氧化酶阴性，过氧化氢酶阳性。对16S rRNA基因的系统发育分析表明，菌株DY1(T)属于嗜放射菌属，与水生嗜放射菌CCUG 53370(T)（序列相似性为96.2%）和纳瓦霍嗜放射菌KR - 114(T)（序列相似性为94.1%）具有序列相似性。菌株DY1(T)与水生嗜放射菌CCUG 53370(T)的DNA相关性较低（41.3 ± 3.9%）。DY1(T)的DNA G + C含量为58.7 mol%。主要脂肪酸为特征性总和3（C16:1 ω7c/ω6c）、C16:0和C17:0。肽聚糖中的主要氨基酸为D - 丙氨酸、L - 谷氨酸、甘氨酸和L - 鸟氨酸。主要极性脂类为未知的磷酸糖脂（PGL）。菌株DY1(T)对伽马辐射具有抗性，被发现是一个新物种。因此，该菌株被命名为DY1(T)（=KCTC 33027(T) = JCM 18576(T)），并在此提出新种深红嗜放射菌的名称。
