Asker Dalal, Awad Tarek S, Beppu Teruhiko, Ueda Kenji
Department of Food Science, University of Massachusetts, 100 Holdsworth Way, Amherst, MA 01003, USA.
Syst Appl Microbiol. 2008 Mar;31(1):43-9. doi: 10.1016/j.syapm.2007.10.002. Epub 2007 Dec 21.
Two gamma- and UV-radiation resistant, Gram-positive, red- or pink-pigmented, rod-shaped, strictly aerobic, oxidase- and catalase-positive bacterial strains, TDMA-25T and TDMA-uv51T, were isolated from fresh water collected at Misasa, a radioactive site in Japan. Phylogenetic analysis based on 16S rRNA gene sequences placed both in a distinct lineage in the family Deinococcaceae, and the highest degrees of sequence similarity determined belonged to Deinococcus maricopensis LB-34T (88.8-89.3%), Deinococcus pimensis KR-235T (86.4-86.7%) and Deinococcus yavapaiensis KR-236T (86.1%). The DNA G+C content of the strains was 53-58 mol%. The major respiratory quinone was MK-8. The predominant fatty acids were C15:0 iso, C16:0 iso, C13:0 iso, C17:0 iso, C16:0, C13:0 anteiso, C15:0 and C12:0 iso. The strains degraded gelatin, casein, starch and Tween 80. Unique physiological characteristics, differences in their fatty acid profiles, and genotypic and phylogenetic features, differentiated strains TDMA-25T and TDMA-uv51T from closely related Deinococcus species. Hence, the two strains are described as novel species of the genus Deinococcus. The names Deinococcus misasensis sp. nov. (type strain TDMA-25T=JCM 14369=NBRC 102116=CCUG 53610) and Deinococcus roseus sp. nov. (type strain TDMA-uv51T=JCM 14370=NBRC 102117=CCUG 53611) are proposed.
从日本放射性地点三朝采集的淡水中分离出两株抗γ射线和紫外线、革兰氏阳性、呈红色或粉红色、杆状、严格需氧、氧化酶和过氧化氢酶阳性的细菌菌株，TDMA - 25T和TDMA - uv51T。基于16S rRNA基因序列的系统发育分析将二者置于嗜放射菌科的一个独特谱系中，确定的最高序列相似性属于滨海嗜放射菌LB - 34T（88.8 - 89.3%）、皮门斯嗜放射菌KR - 235T（86.4 - 86.7%）和雅瓦派嗜放射菌KR - 236T（86.1%）。这些菌株的DNA G + C含量为53 - 58 mol%。主要呼吸醌为MK - 8。主要脂肪酸为异C15:0、异C16:0、异C13:0、异C17:0、C16:0、反异C13:0、C15:0和异C12:0。这些菌株可降解明胶、酪蛋白、淀粉和吐温80。独特的生理特征、脂肪酸谱差异以及基因型和系统发育特征，使TDMA - 25T和TDMA - uv51T菌株与密切相关的嗜放射菌属物种区分开来。因此，这两株菌株被描述为嗜放射菌属的新物种。提出了新种三朝嗜放射菌（模式菌株TDMA - 25T = JCM 14369 = NBRC 102116 = CCUG 53610）和玫瑰嗜放射菌（模式菌株TDMA - uv51T = JCM 14370 = NBRC 102117 = CCUG 53611）的名称。
