支链RNA的化学合成：一种基于空间控制利用2'-磷酸化构建支链RNA合成单元的新方法。

Chemical synthesis of branched RNAs: a new method for construction of synthetic units for branched RNAs by use of 2'-phosphorylation on the basis of steric control.

作者信息

Sekine M, Iimura S, Mori T, Tsuruoka H, Furusawa K

机构信息

Department of Life Science, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.

出版信息

Nucleic Acids Symp Ser. 1991(25):65-6.

PMID:1726812

Abstract

A 3',5'-unprotected 2'-O-bis(tert-butoxy)-phosphinyl-6-N-benzoyladenosine derivative was prepared as a key intermediate for the synthesis of branched RNAs, and used for construction of building units from which chain elongation in any of 2'-, 3'-, and 5'-directions would be possible. From this synthetic intermediate, 2'-phosphorylated ApC dimer was also synthesized.

摘要

制备了一种3'，5'-未保护的2'-O-双（叔丁氧基）-磷酰基-6-N-苯甲酰腺苷衍生物作为合成支链RNA的关键中间体，并用于构建能够在2'-、3'-和5'-任一方向进行链延伸的构建单元。从这种合成中间体还合成了2'-磷酸化的ApC二聚体。