Z-RNA。核糖-CGCGCG的2'-O-[13C]甲基和5-甲基类似物的合成。

Z-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.

作者信息

Biała E, Milecki J, Kowalewski A, Popenda M, Antkowiak W Z, Adamiak R W

机构信息

Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań.

出版信息

Acta Biochim Pol. 1993;40(4):521-30.

PMID:7511312

Abstract

Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.

摘要

介绍了使用载体辅助亚磷酰胺法化学合成2'-O-[¹³C]甲基-rCGCGCG和5-甲基-rCGCGCG的方法。通过用1,3-二氯-1,1,3,3-四异丙基二硅氧烷（马尔凯维茨试剂）进行瞬时5',3'-O-硅烷化，将2'-O-甲基鸟苷衍生物与其3'-O-甲基对应物分离。得到了纯度适合核磁共振研究的六聚体。