Khezri Shiva, Valojerdi Mojtaba R, Sepehri Houri, Baharvand Hossein
Department of Stem Cells, Royan Institute, Tehran, Iran.
Saudi Med J. 2007 Feb;28(2):181-6.
To investigate the effect of basic fibroblast growth factor (bFGF) on the differentiation of embryonic stem cells (ESCs) into early cardiomyocytes.
Embryoid bodies (EBs) were produced from mouse ESC line (Royan B1) in hanging drops and cultured for 5 days as suspension. During the first 2 days of suspension, the EBs of the experimental group were treated with 10 ng/ml of bFGF and subsequently plated onto gelatin-coated tissue culture dishes (day 7). The differentiated cells were evaluated pharmacologically, by immunocytochemistry, and so forth. The study was carried out in the Department of Stem Cells, Royan Institute, Tehran, Iran in 2005.
The beating frequency in the bFGF treated EBs was less than that in the control group. In addition, the beating in the EBs of the experimental group, treated with isoprenaline and phenylephrine, was only more than 7+3 days in comparison to the control group. The response of the EBs to carbachol was more in the bFGF group than 7+14 days. In all the stages of development, the beating cells in the EBs of both groups expressed beta-actinin, myosin light chain isoform 2V, cardiac alpha-myosin heavy chain (alpha-MHC), and cardiac beta-myosin heavy chain (beta-MHC). Nonetheless, during 7+3 days, the last 2 genes were more advanced in the bFGF group. The atrial natriuretic factor was also expressed at a late stage in both groups.
Basic fibroblast growth factor can only promote the early maturation of ESC-derived cardiomyocytes in terms of chronotropic characteristics and expression of cardiac alpha-MHC and beta-MHC.
研究碱性成纤维细胞生长因子（bFGF）对胚胎干细胞（ESC）分化为早期心肌细胞的影响。
从小鼠胚胎干细胞系（Royan B1）通过悬滴法制备胚状体（EBs），并作为悬浮液培养5天。在悬浮培养的前2天，实验组的EBs用10 ng/ml的bFGF处理，随后接种到明胶包被的组织培养皿上（第7天）。通过药理学、免疫细胞化学等方法对分化细胞进行评估。该研究于2005年在伊朗德黑兰的Royan研究所干细胞系进行。
bFGF处理的EBs的搏动频率低于对照组。此外，与对照组相比，实验组用异丙肾上腺素和去氧肾上腺素处理的EBs的搏动仅超过7 + 3天。bFGF组的EBs对卡巴胆碱的反应比对照组多7 + 14天。在发育的所有阶段，两组EBs中的搏动细胞均表达β - 肌动蛋白、肌球蛋白轻链同工型2V、心脏α - 肌球蛋白重链（α - MHC）和心脏β - 肌球蛋白重链（β - MHC）。然而，在7 + 3天期间，bFGF组中后两个基因的表达更先进。两组在后期均表达心房利钠因子。
碱性成纤维细胞生长因子仅能在变时特性以及心脏α - MHC和β - MHC的表达方面促进ESC来源的心肌细胞的早期成熟。
