黄瓜（Cucumis sativus L.）经细胞培养传代后镶嵌体（MSC）表型的选择——一种获得植物线粒体突变体的方法。
The selection of mosaic (MSC) phenotype after passage of cucumber (Cucumis sativus L.) through cell culture - a method to obtain plant mitochondrial mutants.
作者信息
Bartoszewski Grzegorz, Havey Michael J, Ziółkowska Agnieszka, Długosz Marek, Malepszy Stefan
机构信息
Department of Plant Genetics, Breeding and Biotechnology, Faculty of Horticulture and Landscape Architecture, Warsaw Agricultural University, Nowoursynowska 159, 02-776 Warszawa, Poland.
出版信息
J Appl Genet. 2007;48(1):1-9. doi: 10.1007/BF03194652.
Mosaic (MSC) mutants of cucumber (Cucumis sativus L.) appear after passage through cell cultures. The MSC phenotype shows paternal transmission and is associated with mitochondrial DNA rearrangements. This review describes the origins and phenotypes of independently produced MSC mutants of cucumber, including current knowledge on their mitochondrial DNA rearrangements, and similarities of MSC with other plant mitochondrial mutants. Finally we propose that passage of cucumber through cell culture can be used as a unique and efficient method to generate mitochondrial mutants of a higher plant in a highly homozygous nuclear background.
黄瓜（Cucumis sativus L.）的花叶镶嵌体（MSC）突变体在经过细胞培养后出现。MSC表型显示父系遗传，并与线粒体DNA重排有关。本文综述了独立产生的黄瓜MSC突变体的起源和表型，包括其线粒体DNA重排的现有知识，以及MSC与其他植物线粒体突变体的相似性。最后，我们提出将黄瓜进行细胞培养可作为一种独特且高效的方法，在高度纯合的核背景下产生高等植物的线粒体突变体。
Zotero 插件