Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211.
Plant Physiol. 1991 May;96(1):232-8. doi: 10.1104/pp.96.1.232.
The nonchromosomal stripe 2 (NCS2) mutant of maize (Zea mays L.) has a DNA rearrangement in the mitochondrial genome that segregates with the abnormal growth phenotype. Yet, the NCS2 characteristic phenotype includes striped sectors of pale-green tissue on the leaves. This suggests a chloroplast abnormality. To characterize the chloroplasts present in the mutant sectors, we examined the chloroplast structure by electron microscopy, chloroplast function by radiolabeled carbon dioxide fixation and fluorescence induction kinetics, and thylakoid protein composition by polyacrylamide gel electrophoresis. The data from these analyses suggest abnormal or prematurely arrested chloroplast development. Deleterious effects of the NCS2 mutant mitochondria upon the cells of the leaf include structural and functional alterations in the both the bundle sheath and mesophyll chloroplasts.
玉米（Zea mays L.）的非染色体条纹 2（NCS2）突变体在线粒体基因组中有一个 DNA 重排，与异常生长表型分离。然而，NCS2 特征表型包括叶片上淡绿色组织的条纹状区域。这表明叶绿体异常。为了描述突变体组织中存在的叶绿体，我们通过电子显微镜检查了叶绿体结构，通过放射性标记的二氧化碳固定和荧光诱导动力学检查了叶绿体功能，并通过聚丙烯酰胺凝胶电泳检查了类囊体蛋白组成。这些分析的数据表明叶绿体发育异常或过早停滞。NCS2 突变体线粒体对叶片细胞的有害影响包括束鞘和叶肉叶绿体的结构和功能改变。
