Havey M J, Park Y H, Bartoszewski G
Vegetable Crops Unit, Agricultural Research Service, US Department of Agriculture, Department of Horticulture, 1575 Linden Dr., University of Wisconsin, Madison, WI 53706, USA.
J Hered. 2004 Nov-Dec;95(6):492-7. doi: 10.1093/jhered/esh081.
The mitochondrial genome of cucumber shows paternal transmission and there are no reports of variation for mitochondrial transmission in cucumber. We used a mitochondrially encoded mosaic (MSC) phenotype to reveal phenotypic variation for mitochondrial-genome transmission in cucumber. At least 10 random plants from each of 71 cucumber plant introductions (PIs) were crossed as the female with an inbred line (MSC16) possessing the MSC phenotype. Nonmosaic F1 progenies were observed at high frequencies (greater than 50%) in F1 families from 10 PIs, with the greatest proportions being from PI 401734. Polymorphisms near the mitochondrial cox1 gene and JLV5 region revealed that nonmosaic hybrid progenies from crosses of PI 401734 with MSC16 as the male possessed the nonmosaic-inducing mitochondrial DNA (mtDNA) from the paternal parent. F2) F3, and backcross progenies from nonmosaic F1 plants from PI 401734 x MSC16 were testcrossed with MSC16 as the male parent to reveal segregation of a nuclear locus (Psm for Paternal sorting of mitochondria) controlling sorting of mtDNA from the paternal parent. Psm is a unique locus at which the maternal genotype affects sorting of paternally transmitted mtDNA.
黄瓜的线粒体基因组表现出父系遗传，且尚无关于黄瓜线粒体遗传变异的报道。我们利用线粒体编码的嵌合（MSC）表型来揭示黄瓜线粒体基因组遗传的表型变异。将71份黄瓜种质资源（PIs）中的至少10株随机植株作为母本与具有MSC表型的自交系（MSC16）杂交。在来自10个PIs的F1家系中，非嵌合F1后代出现的频率很高（超过50%），其中比例最高的来自PI 401734。线粒体cox1基因和JLV5区域附近的多态性表明，以MSC16为父本、PI 401734为母本杂交产生的非嵌合杂交后代拥有来自父本的非嵌合诱导线粒体DNA（mtDNA）。将来自PI 401734×MSC16的非嵌合F1植株的F2、F3和回交后代与作为父本的MSC16进行测交，以揭示控制来自父本的mtDNA分选的一个核基因座（线粒体父本分选的Psm）的分离情况。Psm是一个独特的基因座，其母本基因型会影响父系传递的mtDNA的分选。
