Yu L, Zhao J, Zhu Q, Li S P
National Standard Lab for Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, China.
J Pharm Biomed Anal. 2007 Jun 28;44(2):439-43. doi: 10.1016/j.jpba.2007.01.003. Epub 2007 Jan 9.
A method, namely macrophage biospecific extraction and high performance liquid chromatography for screening potential immunological active components in Cordyceps sinensis, a well-known traditional Chinese medicine, was developed. Two components, which could interact with macrophage, in aqueous extract of C. sinensis (WECS) were found by comparing the HPLC chromatograms of WECS before and after interacted with macrophage. The two compounds were identified as guanosine and adenosine. Their effects on mice macrophage were also investigated in vitro. The results showed that adenosine and guanosine could attenuate NO (p<0.01) but augment interleukin-lbeta (IL-1beta) (p<0.05) release of macrophage during the tested concentrations. In addition, guanosine (0.10 micromol/ml) also increased alpha-tumor necrosis factor (TNF-alpha) release of macrophage. The data suggest that macrophage biospecific extraction and HPLC is a useful method to screen immunological active components from Chinese medicines.
开发了一种用于筛选著名中药冬虫夏草中潜在免疫活性成分的方法，即巨噬细胞生物特异性提取和高效液相色谱法。通过比较冬虫夏草水提取物（WECS）与巨噬细胞相互作用前后的高效液相色谱图，发现了两种能与巨噬细胞相互作用的成分。这两种化合物被鉴定为鸟苷和腺苷。还在体外研究了它们对小鼠巨噬细胞的影响。结果表明，在所测试的浓度范围内，腺苷和鸟苷可减弱一氧化氮（p<0.01）的释放，但增强巨噬细胞白细胞介素-1β（IL-1β）（p<0.05）的释放。此外，鸟苷（0.10微摩尔/毫升）还增加了巨噬细胞α-肿瘤坏死因子（TNF-α）的释放。数据表明，巨噬细胞生物特异性提取和高效液相色谱法是从中药中筛选免疫活性成分的一种有用方法。
