Ahn H, Shin H, Yun S, Kim J, Choi J
Department of Biomedical Engineering, Graduate School, Yeungnam University, Daegu, Korea; Research Institutes of Biomedical Engineering, Yeungnam University, Daegu, Korea.
Conf Proc IEEE Eng Med Biol Soc. 2005;2005:1945-7. doi: 10.1109/IEMBS.2005.1616833.
During liver resection and liver transplant, liver is damaged by ischemia-reperfusion injury. Until now, there is no approved method to measure or predict the extent of liver injury during the operation. This is the preliminary study to make the real time monitoring system by quantification of bioimpedance and ischemiareperfusion reperfusion injury in liver. Sprague-Dawley rats were subjected to different periods of 70% partial hepatic ischemia (30, 60, 90 and 120minutes ischemia) and reperfusion. We measured changes of liver tissue bioimpedance (120Hz-100KHz) every five minutes. Cell viability was assessed by metabolic capacity of fatty acid (palmitic acid metabolic rate), ATP content and histological examination (H/E and TUNEL stain) at every 30 minutes interval during ischemia.
在肝切除和肝移植过程中，肝脏会受到缺血再灌注损伤。到目前为止，尚无经批准的方法来测量或预测手术期间肝损伤的程度。这是一项通过定量生物阻抗和肝脏缺血再灌注损伤来建立实时监测系统的初步研究。将Sprague-Dawley大鼠进行不同时长（30、60、90和120分钟缺血）的70%部分肝缺血及再灌注处理。我们每隔五分钟测量肝组织生物阻抗（120Hz - 100KHz）的变化。在缺血期间，每隔30分钟通过脂肪酸代谢能力（棕榈酸代谢率）、ATP含量和组织学检查（苏木精/伊红染色和TUNEL染色）评估细胞活力。
