Kato Kiyoko, Yoshimoto Momoko, Kato Keiji, Adachi Sawako, Yamayoshi Asako, Arima Takahiro, Asanoma Kazuo, Kyo Satoru, Nakahata Tatsutoshi, Wake Norio
Department of Molecular Genetics, Division of Molecular and Cell Therapeutics, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka, Japan.
Hum Reprod. 2007 May;22(5):1214-23. doi: 10.1093/humrep/del514. Epub 2007 Feb 5.
It has been proposed that the human endometrium may contain a population of adult stem cells that are responsible for its remarkable regenerative capability. Recently, a subset of stem cells or progenitor cells in adult tissue has been identified as side-population cells (SP cells) displaying low staining with Hoechst 33342 by fluorescence-activated cell sorter (FACS) analysis. In this study, we isolated SP cells from the human endometrium and analysed their properties.
Endometrial cells were obtained using enzymatic digestion from uterine hysterectomy for the treatment of uterine myoma and stained with Hoechst 33342 dye either alone or in combination with verapamil. The cells were then analysed using FACS.
SP cells were present among normal human endometrial cells. Most SP cells were enriched in the CD9(-)CD13(-) fraction. These SP cells showed long-term repopulating properties and produced gland (CD9(+))- and stroma (CD13(+))-like cells. CD9(-)CD13(-) cells isolated from the endometrium also generated gland- or stroma-like cells.
SP cells in the human endometrium can function as progenitor cells. This is the first report of the phenotype of SP cells from normal human endometrial cells.
有人提出，人类子宫内膜可能含有一群成年干细胞，它们负责子宫内膜显著的再生能力。最近，成年组织中的一部分干细胞或祖细胞已被鉴定为侧群细胞（SP细胞），通过荧光激活细胞分选仪（FACS）分析，这些细胞对Hoechst 33342染色较低。在本研究中，我们从人类子宫内膜中分离出SP细胞并分析其特性。
通过酶消化从因子宫肌瘤接受子宫切除术的子宫中获取子宫内膜细胞，单独或与维拉帕米联合用Hoechst 33342染料染色。然后使用FACS分析这些细胞。
正常人类子宫内膜细胞中存在SP细胞。大多数SP细胞富集于CD9(-)CD13(-)组分中。这些SP细胞表现出长期增殖特性，并产生腺体样（CD9(+)）和基质样（CD13(+)）细胞。从子宫内膜分离出的CD9(-)CD13(-)细胞也能产生腺体样或基质样细胞。
人类子宫内膜中的SP细胞可作为祖细胞发挥作用。这是关于正常人类子宫内膜细胞中SP细胞表型的首次报道。
