Department of Obstetrics and Gynecology, Faculty of Medicine, Juntendo University, Hongo 2-1-1, Bunkyo-ku, Tokyo 113-8421, Japan.
Anticancer Res. 2012 Jun;32(6):2283-93.
Adult stem cells have recently been identified in several types of mature tissue and it has also been suggested that stem-like cells exist in cancerous tissues. In this regard, stem-like cell subpopulations, referred to as side-population (SP) cells, have been identified in several tissue and tumor types, based on their ability to remove intracellular Hoechst 33342, a fluorescent dye. We have isolated and characterized SP cells from normal human endometrium and an endometrial cancer cell line. Endometrial SP cells can function as progenitor cells. Endometrial cancer SP cells possess the following characteristics: i) reduced expression levels of differentiation markers, ii) long-term repopulating properties, iii) self-renewal capacity, iv) enhanced migration and podia formation, v) enhanced tumorigenicity, and vi) bi-potential development (tumor cells and stroma-like cells), suggesting that they have cancer stem-like cell features. Recently, we demonstrated that sodium butyrate, a histone deacetylase (HDAC) inhibitor, inhibited the self-renewal capacity of endometrial cancer SP cells by inducing a DNA damage response. Here, we review recent articles that show the presence of stem cells in normal endometrium and endometrial cancer and introduce the results of our own recent studies.
成人干细胞最近在几种成熟组织中被发现，也有人提出，在癌组织中存在类似干细胞的细胞。在这方面，基于其去除细胞内 Hoechst 33342（一种荧光染料）的能力，已在几种组织和肿瘤类型中鉴定出称为侧群 (SP) 细胞的类似干细胞的细胞亚群。我们已经从正常人类子宫内膜和子宫内膜癌细胞系中分离和表征了 SP 细胞。子宫内膜 SP 细胞可以作为祖细胞发挥功能。子宫内膜癌 SP 细胞具有以下特征：i）分化标志物表达水平降低，ii）长期重编程特性，iii）自我更新能力，iv）增强的迁移和伪足形成，v）增强的致瘤性，以及 vi）双潜能发育（肿瘤细胞和基质样细胞），表明它们具有类似癌症干细胞的特征。最近，我们证明了组蛋白去乙酰化酶 (HDAC) 抑制剂丁酸钠通过诱导 DNA 损伤反应抑制子宫内膜癌 SP 细胞的自我更新能力。在这里，我们回顾了最近的文章，这些文章表明在正常子宫内膜和子宫内膜癌中存在干细胞，并介绍了我们自己最近的研究结果。
