[鼓室内注射庆大霉素后庆大霉素在内耳的分布]
[Distribution of gentamicin in inner ear after intratympanic gentamicin injection].
作者信息
Liu Jian-Ping, Dai Chun-Fu, Wang Zheng-Min, Chi Fang-Lu, Tian Jie, Da Cui-Di
机构信息
Department of Otology and Skull Base, Eye Ear Nose and Throat Hospital, Fudan University, Shanghai 200031, China.
出版信息
Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2006 Nov;41(11):851-6.
OBJECTIVE
To investigate uptake and accumulation of gentamicin by cells in the guinea pig inner ear after intratympanic injection using a fluorescent probe--gentamicin-Texas-red conjunction (GTTR).
METHODS
Adult guinea pigs (n = 80) were administered a single dose of GTrR to the middle ear cavity through the intact membrane and survived for 12 h, 24 h, 48 h, 3 d, 4 d, 7 d, 14 d and 28 d. The distribution of GTTR in the cochlear and vestibular cells was observed after staining with phalloidin-alexa-488. Texas Red and DMSO were injected into the tympanum as control.
RESULTS
Diffuse staining of gentamicin in the labyrinth was observed initially after local drug administration. At later time point the outer hair cells and sensory cells of vestibular organ were staining more densely than the support cells in the inner ear. The peak level of fluorescent density was reached 3 days after local injection. The GTTR was observed in the infracuticular zone.
CONCLUSIONS
GTTR was a potential fluorescent probe to investigate the pharmacokinetics and mechanisms of gentamicin accumulation in local application.
目的
使用荧光探针——庆大霉素-德克萨斯红偶联物（GTTR），研究鼓膜内注射后豚鼠内耳细胞对庆大霉素的摄取和蓄积情况。
方法
成年豚鼠（n = 80）通过完整鼓膜向中耳腔单次注射GTrR，并分别存活12小时、24小时、48小时、3天、4天、7天、14天和28天。用鬼笔环肽-亚历克萨-488染色后，观察GTTR在耳蜗和前庭细胞中的分布。将德克萨斯红和二甲基亚砜注入鼓室作为对照。
结果
局部给药后最初在迷路中观察到庆大霉素的弥漫性染色。在随后的时间点，内耳的外毛细胞和前庭器官的感觉细胞比支持细胞染色更密集。局部注射后3天达到荧光密度峰值。在表皮下区域观察到GTTR。
结论
GTTR是一种潜在的荧光探针，可用于研究庆大霉素局部应用的药代动力学和蓄积机制。
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