Partlow Kathryn C, Chen Junjie, Brant Jason A, Neubauer Anne M, Meyerrose Todd E, Creer Michael H, Nolta Jan A, Caruthers Shelton D, Lanza Gregory M, Wickline Samuel A
Department of Medicine, Washington University School of Medicine, 660 South Euclid Ave., St. Louis, MO 63110, USA.
FASEB J. 2007 Jun;21(8):1647-54. doi: 10.1096/fj.06-6505com. Epub 2007 Feb 6.
MRI has been employed to elucidate the migratory behavior of stem/progenitor cells noninvasively in vivo with traditional proton (1H) imaging of iron oxide nanoparticle-labeled cells. Alternatively, we demonstrate that fluorine (19F) MRI of cells labeled with different types of liquid perfluorocarbon (PFC) nanoparticles produces unique and sensitive cell markers distinct from any tissue background signal. To define the utility for cell tracking, mononuclear cells harvested from human umbilical cord blood were grown under proendothelial conditions and labeled with nanoparticles composed of two distinct PFC cores (perfluorooctylbromide and perfluoro-15-crown-5 ether). The sensitivity for detecting and imaging labeled cells was defined on 11.7T (research) and 1.5T (clinical) scanners. Stem/progenitor cells (CD34+ CD133+ CD31+) readily internalized PFC nanoparticles without aid of adjunctive labeling techniques, and cells remained functional in vivo. PFC-labeled cells exhibited distinct 19F signals and were readily detected after both local and intravenous injection. PFC nanoparticles provide an unequivocal and unique signature for stem/progenitor cells, enable spatial cell localization with 19F MRI, and permit quantification and detection of multiple fluorine signatures via 19F MR spectroscopy. This method should facilitate longitudinal investigation of cellular events in vivo for multiple cell types simultaneously.
磁共振成像（MRI）已被用于通过对氧化铁纳米颗粒标记的细胞进行传统的质子（1H）成像，在体内非侵入性地阐明干细胞/祖细胞的迁移行为。另外，我们证明，对用不同类型的液体全氟化碳（PFC）纳米颗粒标记的细胞进行氟（19F）MRI检查，可产生独特且灵敏的细胞标记物，与任何组织背景信号都不同。为了确定细胞追踪的实用性，从人脐带血中采集的单核细胞在促内皮细胞条件下培养，并用由两种不同的PFC核（全氟辛基溴和全氟-15-冠-5醚）组成的纳米颗粒进行标记。在11.7T（研究型）和1.5T（临床型）扫描仪上确定了检测和成像标记细胞的灵敏度。干细胞/祖细胞（CD34 + CD133 + CD31 +）无需辅助标记技术即可轻松内化PFC纳米颗粒，并且细胞在体内仍保持功能。PFC标记的细胞表现出独特的19F信号，在局部和静脉注射后均易于检测到。PFC纳米颗粒为干细胞/祖细胞提供了明确且独特的特征，能够通过19F MRI进行空间细胞定位，并允许通过19F磁共振波谱对多个氟特征进行定量和检测。该方法应有助于同时对多种细胞类型的体内细胞事件进行纵向研究。
