de Moraes Pantaleão Silmara, Alcântara Ayda Vera, Hora Alves José do Patrocínio, Pavanin Luiz Alfredo, Graf Ulrich, de Rezende Alexandre Azenha Alves, Bueno Valadares Bruno Lassmar, Fragiorge Edson José, de Souza Neila Coelho, Guterres Zaira da Rosa, Spanó Mário Antoônio
Departamento de Biologia, Universidade Federal de Sergipe, São Cristóvão, Estado de Sergipe, SE, Brazil.
Environ Mol Mutagen. 2007 Mar;48(2):96-105. doi: 10.1002/em.20281.
The Drosophila melanogaster somatic mutation and recombination test (SMART) was used to assess the genotoxicity of surface (S) and bottom (B) water and sediment samples collected from Sites 1 and 2 on the Japaratuba River (Sergipe, Brazil), an area impacted by a petrochemical industrial complex that indirectly discharges treated effluent (produced water) into the river. The genotoxicity tests were performed in standard (ST) cross and high bioactivation (HB) cross flies and were conducted on samples taken in March (dry season) and in July (rainy season) of 2003. Mutant spot frequencies found in treatments with unprocessed water and sediment samples from the test sites were compared with the frequencies observed for similar samples taken from a clean reference site (the Jacarecica River in Sergipe, Brazil) and those of negative (ultrapure water) controls. While samples from the Japaratuba River generally produced greater responses than those from the Jacarecica River, positive responses were detected for both the test and reference site samples. All the water samples collected in March 2003 were genotoxic. In July 2003, the positive responses were restricted to water samples collected from Sites 1 B and 2 S in the ST cross. The genotoxicity of the water samples was due to mitotic recombination, and the samples produced similar genotoxic responses in ST and HB flies. The spot frequencies found in the July water samples were considerably lower than those for the March water samples, suggesting a seasonal effect. The only sediment samples that were genotoxic were from Site 1 (March and July) and from the Jacarecica River (March). The genotoxins in these samples produced both somatic mutation (limited to the Site 1 sample in HB flies) and recombination. The results of this study indicate that samples from both the Japaratuba and Jacarecica Rivers were genotoxic, with the most consistently positive responses detected with Site 1 samples, the site closest to the putative pollution source.
利用黑腹果蝇体细胞突变和重组试验（SMART）评估了从雅帕拉图巴河（巴西塞尔希培州）1号和2号采样点采集的地表水（S）、底水（B）和沉积物样本的遗传毒性。该区域受一个石化工业园区影响，该园区间接将处理后的废水（采出水）排放到河中。遗传毒性试验在标准（ST）杂交和高生物活化（HB）杂交果蝇中进行，试验样本采集于2003年3月（旱季）和7月（雨季）。将试验地点未经处理的水和沉积物样本处理后的突变斑频率与从清洁参考地点（巴西塞尔希培州的雅卡雷西卡河）采集的类似样本以及阴性（超纯水）对照的频率进行比较。虽然雅帕拉图巴河的样本通常比雅卡雷西卡河的样本产生更大的反应，但试验和参考地点的样本均检测到阳性反应。2003年3月采集的所有水样均具有遗传毒性。2003年7月，阳性反应仅限于ST杂交中从1B号和2S号采样点采集的水样。水样的遗传毒性是由于有丝分裂重组引起的，这些样本在ST和HB果蝇中产生了类似的遗传毒性反应。7月水样中的斑频率明显低于3月水样，表明存在季节效应。唯一具有遗传毒性的沉积物样本来自1号采样点（3月和7月）和雅卡雷西卡河（3月）。这些样本中的遗传毒素既产生了体细胞突变（仅限于HB果蝇中的1号采样点样本），也产生了重组。这项研究的结果表明，雅帕拉图巴河和雅卡雷西卡河的样本均具有遗传毒性，其中1号采样点（最靠近假定污染源的地点）的样本检测到的阳性反应最为一致。
Zotero 插件