[虫草多糖对二甲基亚硝胺诱导的大鼠肝纤维化脂质过氧化的影响]

[Effect of cordyceps polysaccharide on lipid peroxidation of rats with dimethylnitrosamine-induced liver fibrosis].

作者信息

Peng Jing-Hua, Li Xue-Mei, Hu Yi-Yang, Feng Qin

机构信息

Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Institute of Liver Disease of Shanghai University of Traditional Chinese Medicine, E-Research Institute of Internal Medicine of Traditional Chinese Medicine for Universities in Shanghai, Shanghai 201203, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2013 Feb;38(3):391-6.

PMID:23668016

Abstract

OBJECTIVE

To observe the pharmacological effect of Cordyceps polysaccharide on dimethylnitrosamine (DMN)-induced liver fibrosis in rats.

METHOD

DMN rat liver fibrosis model was established and divided into the normal group (N, n = 6), the model group (M, n = 11), the Cordyceps polysaccharide group (C, n = 8) and the colchicine group (Q, n = 9). During the modeling for four weeks, Cordyceps polysaccharide (60 mg x kg(-1)) and colchicine (0.1 mg x kg(-1)) were orally administered for three weeks, while the model and normal groups were given disinfected water of the same amount.

OBSERVATION

serum ALT, AST, GGT and Alb, TBil content; content of hydroxyproline (Hyp) in liver tissues; liver pathology and collagen staining; SOD activity and MDA, GSH, GSH-Px in liver tissues; protein expression of proliferating cell nuclear antigen (PCNA) in liver tissues.

RESULT

Serum ALT, AST, GGT, TBil significantly increased, and A1b decreased significantly in the model group. Hepatic Hyp significantly increased in the model group, whereas the index remarkably decreased in the Cordyceps polysaccharide group and the colchicine group. HE staining: the structure of normal hepatic lobules was damaged, with hepatocytes tumefaction and proliferation of connective tissues in portal tracts in the model group, while the Cordyceps polysaccharide group and the colchicine group recorded notable reduction in above pathological changes. Collagen staining: the model group showed hepatic lobule fibrous septum and many intact pseudolobules; while the Cordyceps polysaccharide group and the colchicine group witnessed decrease in collagen deposition. The model group showed significant decrease in SOD, GSH-Px and GSH and increase in MDA, whereas the Cordyceps polysaccharide group and the colchicine group recorded notable growth in GSH and GSH-Px. The model group showed significant decrease in protein expression of PCNA in liver tissues, while the Cordyceps polysaccharide group and the colchicine group showed significant reduction.

CONCLUSION

Cordyceps polysaccharide can significantly inhibit DMN-induced liver fibrosis and lipid peroxidation in rats.

摘要

目的

观察虫草多糖对二甲基亚硝胺（DMN）诱导的大鼠肝纤维化的药理作用。

方法

建立DMN大鼠肝纤维化模型，分为正常组（N，n = 6）、模型组（M，n = 11）、虫草多糖组（C，n = 8）和秋水仙碱组（Q，n = 9）。在造模四周期间，虫草多糖（60 mg·kg⁻¹）和秋水仙碱（0.1 mg·kg⁻¹）灌胃给药三周，模型组和正常组给予等量消毒水。

观察指标

血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、γ-谷氨酰转肽酶（GGT）及白蛋白（Alb）、总胆红素（TBil）含量；肝组织羟脯氨酸（Hyp）含量；肝脏病理及胶原染色；肝组织超氧化物歧化酶（SOD）活性及丙二醛（MDA）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）含量；肝组织增殖细胞核抗原（PCNA）蛋白表达。

结果

模型组血清ALT, AST, GGT, TBil显著升高，Alb显著降低。模型组肝组织Hyp显著升高，而虫草多糖组和秋水仙碱组该指标显著降低。苏木精-伊红（HE）染色：模型组正常肝小叶结构破坏，肝细胞肿胀，汇管区结缔组织增生，而虫草多糖组和秋水仙碱组上述病理改变明显减轻。胶原染色：模型组肝小叶纤维间隔形成，可见多个完整假小叶；而虫草多糖组和秋水仙碱组胶原沉积减少。模型组SOD、GSH-Px和GSH显著降低，MDA升高，而虫草多糖组和秋水仙碱组GSH和GSH-Px显著升高。模型组肝组织PCNA蛋白表达显著降低，而虫草多糖组和秋水仙碱组显著升高。