Dummer R, Posseckert G, Nestle F, Witzgall R, Burger M, Becker J C, Schäfer E, Wiede J, Sebald W, Burg G
Department of Dermatology, University of Würzburg Medical School, Federal Republic of Germany.
J Invest Dermatol. 1992 Jan;98(1):50-4. doi: 10.1111/1523-1747.ep12494223.
In patients with cutaneous T-cell lymphomas (CTCL), soluble interleukin-2 receptor serum levels (sIL-2R) were determined by ELISA technique, and natural killer cell (NK) activity, by a 4-h chromium-51 release assay. Decrease of NK activity correlated with the augmentation of serum sIL-2R. After a 4-d stimulation with interleukin 2 CTCL patients' peripheral mononuclear cells (PMC) showed an increase of cytotoxic activity similar to that in healthy donors' PMC. Normal donors' PMC demonstrated a diminished IL-2-induced cytotoxic activity in 25% CTCL serum (sIL-2R of 3000, 7330, and 10700 U/ml, respectively) compared to control serum (sIL-2R of 400, 340, and 420 U/ml, respectively). IL-2-dependent proliferation of 2-d phytohemagglutinin (PHA) blasts was lower in CTCL serum than in control serum. sIL-2R was enriched from one CTCL patient's serum by IL-2 affinity chromatography. Transfection of the Tac gene into NIH/3T3 fibroblasts resulted in the production of a recombinant sIL-2R. The presence of enriched native or recombinant sIL-2R inhibited interleukin-2-dependent generation of cytotoxic activity and PHA blast proliferation. We suggest that elevated sIL-2R levels account for diminished NK activity by neutralizing interleukin 2 in CTCL patients.
在皮肤T细胞淋巴瘤（CTCL）患者中，采用酶联免疫吸附测定（ELISA）技术测定可溶性白细胞介素-2受体血清水平（sIL-2R），并通过4小时51铬释放试验测定自然杀伤细胞（NK）活性。NK活性降低与血清sIL-2R升高相关。用白细胞介素2刺激4天后，CTCL患者外周血单个核细胞（PMC）的细胞毒性活性增加，与健康供体的PMC相似。与对照血清（sIL-2R分别为400、340和420 U/ml）相比，正常供体的PMC在25%的CTCL血清（sIL-2R分别为3000、7330和10700 U/ml）中表现出白细胞介素2诱导的细胞毒性活性降低。CTCL血清中2天植物血凝素（PHA）刺激的淋巴细胞依赖白细胞介素2的增殖低于对照血清。通过白细胞介素2亲和层析从一名CTCL患者的血清中富集sIL-2R。将Tac基因转染到NIH/3T3成纤维细胞中可产生重组sIL-2R。富集的天然或重组sIL-2R的存在抑制了白细胞介素2依赖的细胞毒性活性的产生和PHA刺激的淋巴细胞增殖。我们认为，sIL-2R水平升高通过中和CTCL患者体内的白细胞介素2导致NK活性降低。
