Chun-mao Han, Su-yi Wang, Ping-ping Lai, Hang-hui Cen
The Second Affiliated Hospital of Medicine College, Zhejiang University, Hangzhou, China.
Differentiation. 2007 Apr;75(4):292-8. doi: 10.1111/j.1432-0436.2006.00140.x. Epub 2007 Feb 5.
Human bone marrow-derived mesenchymal stem cells (hMSCs) are a population of pluripotent cells. They can differentiate into different embryonic layer cells as osteoblasts, adipocytes, chondrocytes, myoblasts, neurocytes, etc. However, there are only few reports with regard to differentiate hMSCs into epidermal cells in vitro. In this study, we want to investigate the feasibility of inducing hMSCs into epidermal-like cells under specific medium in vitro. hMSCs in specific inducing medium expressed the early markers of epidermal cell lineage, P63, cytokeratin19 (CK19), the late differentiated marker, the pan-cytokeratin, and another early marker, the beta1-integrin, which up-regulated remarkably in inducing medium. Their morphologies were changed from spindle-like fibroblastic appearances to oblate or irregular shapes under phase contrast microscopy. The hemidesmosome structure was found using the transmission electron microscope. All these data suggested that, under certain conditions, hMSCs have the potential to differentiate into epidermal-like cells. It will be of great accordance in the study of the multipotential property of hMSCs.
人骨髓间充质干细胞（hMSCs）是一群多能细胞。它们能够分化为不同胚层的细胞，如成骨细胞、脂肪细胞、软骨细胞、成肌细胞、神经细胞等。然而，关于体外将hMSCs分化为表皮细胞的报道却很少。在本研究中，我们想要探讨在特定培养基中体外诱导hMSCs分化为表皮样细胞的可行性。处于特定诱导培养基中的hMSCs表达了表皮细胞谱系的早期标志物P63、细胞角蛋白19（CK19）、晚期分化标志物全细胞角蛋白，以及另一种早期标志物β1整合素，其在诱导培养基中显著上调。在相差显微镜下，它们的形态从纺锤状的成纤维细胞外观变为扁平或不规则形状。通过透射电子显微镜发现了半桥粒结构。所有这些数据表明，在特定条件下，hMSCs有分化为表皮样细胞的潜能。这将与hMSCs多能特性的研究高度契合。
