Department of Immunology and Hematology, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran.
Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, 20133, Italy.
Mol Biol Rep. 2024 May 24;51(1):675. doi: 10.1007/s11033-024-09599-y.
Bioscaffolds and cells are two main components in the regeneration of damaged tissues via cell therapy. Umbilical cord stem cells are among the most well-known cell types for this purpose. The main objective of the present study was to evaluate the effect of the pretreatment of the foreskin acellular matrix (FAM) by monophosphoryl lipid A (MPLA) and Lactobacillus casei supernatant (LCS) on the attraction of human umbilical cord mesenchymal stem cells (hucMSC).
The expression of certain cell migration genes was studied using qRT-PCR. In addition to cell migration, transdifferentiation of these cells to the epidermal-like cells was evaluated via immunohistochemistry (IHC) and immunocytochemistry (ICC) of cytokeratin 19 (CK19). The hucMSC showed more tissue tropism in the presence of MPLA and LCS pretreated FAM compared to the untreated control group. We confirmed this result by scanning electron microscopy (SEM) analysis, glycosaminoglycan (GAG), collagen, and DNA content. Furthermore, IHC and ICC data demonstrated that both treatments increase the protein expression level of CK19.
Pretreatment of acellular bioscaffolds by MPLA or LCS can increase the migration rate of cells and also transdifferentiation of hucMSC to epidermal-like cells without growth factors. This strategy suggests a new approach in regenerative medicine.
生物支架和细胞是通过细胞疗法再生受损组织的两个主要组成部分。脐带干细胞是最常用于此目的细胞类型之一。本研究的主要目的是评估单磷酰脂质 A（MPLA）和干酪乳杆菌上清液（LCS）预处理包皮去细胞基质（FAM）对人脐带间充质干细胞（hucMSC）吸引力的影响。
使用 qRT-PCR 研究了某些细胞迁移基因的表达。除了细胞迁移外，还通过细胞角蛋白 19（CK19）的免疫组织化学（IHC）和免疫细胞化学（ICC）评估这些细胞向表皮样细胞的转分化。与未处理的对照组相比，在 MPLA 和 LCS 预处理的 FAM 存在下，hucMSC 表现出更强的组织趋向性。我们通过扫描电子显微镜（SEM）分析、糖胺聚糖（GAG）、胶原和 DNA 含量证实了这一结果。此外，IHC 和 ICC 数据表明，两种处理均增加了 CK19 的蛋白表达水平。
MPLA 或 LCS 预处理脱细胞生物支架可以增加细胞的迁移率，并且在没有生长因子的情况下，还可以将 hucMSC 向表皮样细胞转分化。这种策略为再生医学提供了一种新方法。
