香港活禽市场I类新城疫病毒分离株的特性及实时逆转录-聚合酶链反应检测
Characterization of class I Newcastle disease virus isolates from Hong Kong live bird markets and detection using real-time reverse transcription-PCR.
作者信息
Kim L Mia, King Daniel J, Suarez David L, Wong Chun W, Afonso Claudio L
机构信息
Southeast Poultry Research Laboratory, USDA-ARS, 934 College Station Rd., Athens, GA 30605, USA.
出版信息
J Clin Microbiol. 2007 Apr;45(4):1310-4. doi: 10.1128/JCM.02594-06. Epub 2007 Feb 7.
Abstract
Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.
摘要
美国农业部验证的基质基因实时逆转录聚合酶链反应（RT-PCR）检测方法未检测到从香港活禽市场（LBMs）分离出的新城疫病毒。基于融合基因的系统发育分析，这些病毒与在美国活禽市场和野生水禽中发现的弱毒株I类病毒相关。开发了一种补充基质基因检测方法的替代实时RT-PCR检测方法，以有效检测I类病毒。
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