对在日本分离出的新城疫病毒基因型的系统发育分析。
Phylogenetic analysis of Newcastle disease virus genotypes isolated in Japan.
作者信息
Mase Masaji, Imai Kunitoshi, Sanada Yasuyuki, Sanada Naoko, Yuasa Noboru, Imada Tadao, Tsukamoto Kenji, Yamaguchi Shigeo
机构信息
National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3826-30. doi: 10.1128/JCM.40.10.3826-3830.2002.
Abstract
We genetically analyzed field isolates of the Newcastle disease (ND) virus isolated in Japan from 1930 to 2001. The coding region of the fusion protein was amplified by reverse transcriptase PCR and directly sequenced. Phylogenetic analysis revealed the presence of viruses belonging to six of the eight known genotypes. It can be concluded from this study that ND outbreaks in Japan have been of multiple etiologies. [All sequences used in this study were sent to DDBJ and assigned accession numbers AB 070382 to AB 074042.]
摘要
我们对1930年至2001年在日本分离出的新城疫（ND）病毒的野外分离株进行了基因分析。通过逆转录聚合酶链反应扩增融合蛋白的编码区并直接测序。系统发育分析显示存在属于已知八种基因型中六种的病毒。从这项研究可以得出结论，日本的新城疫疫情有多种病因。[本研究中使用的所有序列已提交给日本DNA数据库（DDBJ），并被赋予登录号AB 070382至AB 074042。]
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