Hsu Ping-I, Chen Chung-Hsuan, Hsieh Chieu-Shia, Chang Wei-Chao, Lai Kwok-Hung, Lo Gin-Ho, Hsu Ping-Ning, Tsay Feng-Woei, Chen Yu-Shan, Hsiao Michael, Chen Hui-Chun, Lu Pei-Jung
Division of Gastroenterology, Department of Internal Medicine, Kaohsiung Veterans General Hospital and National Yang-Ming University, Taiwan.
Clin Cancer Res. 2007 Feb 1;13(3):876-83. doi: 10.1158/1078-0432.CCR-06-1404.
To search for novel disease-specific markers in gastric juice by investigating the protein concentrations and components in gastric juice from patients with various gastroduodenal diseases.
Protein concentrations and pH values in fasting gastric juice were examined in 120 healthy subjects and 39 gastric ulcer, 38 duodenal ulcer, and 31 gastric cancer patients. The protein components in gastric juice were studied by two-dimensional PAGE and mass spectrometric analysis.
Protein concentrations in gastric juice of patients with gastric ulcers and gastric cancer were significantly higher than those in healthy subjects (1.06 and 2.61 mg/mL versus 0.48 mg/mL; P=0.001 and P<0.001, respectively), and duodenal ulcer patients had lower gastric juice protein concentrations compared with healthy subjects (0.26 versus 0.48 mg/mL; P<0.05). Gastric hypoacidity and advanced age were independent factors affecting the protein concentrations in gastric juice with odds ratios of 32.9 (95% confidence interval, 11.8-90.9) and 3.2 (95% confidence interval, 1.3-8.3), respectively. Each electrophoresis images of gastric juice could be classified into one of three patterns: basic band, specific band, or nonspecific band. The frequencies of specific band pattern in healthy subjects, gastric ulcer, duodenal ulcer, and gastric cancer patients were 6%, 42%, 6%, and 93%, respectively. Proteomic analysis revealed that alpha1-antitrypsin precursor was the principal peptide in the specific band.
alpha1-antitrypsin precursor in gastric juice is a novel biomarker for gastric cancer and ulcer. A noninvasive method to obtain gastric juice followed by proteomic analysis may serve as a new tool to screen for gastric malignancies.
通过研究各种胃十二指肠疾病患者胃液中的蛋白质浓度和成分，寻找胃液中新型的疾病特异性标志物。
检测了120名健康受试者以及39名胃溃疡患者、38名十二指肠溃疡患者和31名胃癌患者空腹胃液中的蛋白质浓度和pH值。采用二维聚丙烯酰胺凝胶电泳和质谱分析研究胃液中的蛋白质成分。
胃溃疡和胃癌患者胃液中的蛋白质浓度显著高于健康受试者（分别为1.06和2.61mg/mL，而健康受试者为0.48mg/mL；P=0.001和P<0.001），十二指肠溃疡患者胃液中的蛋白质浓度低于健康受试者（0.26mg/mL对0.48mg/mL；P<0.05）。胃酸过少和高龄是影响胃液中蛋白质浓度的独立因素，优势比分别为32.9（95%置信区间，11.8 - 90.9）和3.2（95%置信区间，1.3 - 8.3）。胃液的每个电泳图像可分为三种模式之一：基本条带、特异性条带或非特异性条带。健康受试者、胃溃疡患者、十二指肠溃疡患者和胃癌患者中特异性条带模式的频率分别为6%、42%、6%和93%。蛋白质组学分析显示，α1 - 抗胰蛋白酶前体是特异性条带中的主要肽段。
胃液中的α1 - 抗胰蛋白酶前体是胃癌和溃疡的新型生物标志物。一种获取胃液后进行蛋白质组学分析的非侵入性方法可能成为筛查胃恶性肿瘤的新工具。
