• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

使用串联质谱标签定量技术对人唾液进行差异蛋白质组学分析以检测胃癌

Differential Proteomic Analysis of Human Saliva using Tandem Mass Tags Quantification for Gastric Cancer Detection.

作者信息

Xiao Hua, Zhang Yan, Kim Yong, Kim Sung, Kim Jae Joon, Kim Kyoung Mee, Yoshizawa Janice, Fan Liu-Yin, Cao Cheng-Xi, Wong David T W

机构信息

State Key Laboratory of Microbial Metabolism, Laboratory of Bioseparation and Analytical Biochemistry, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.

School of Pharmacy, Shanghai Jiao Tong University, Shanghai, 200240, China.

出版信息

Sci Rep. 2016 Feb 25;6:22165. doi: 10.1038/srep22165.

DOI:10.1038/srep22165
PMID:26911362
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4766442/
Abstract

Novel biomarkers and non-invasive diagnostic methods are urgently needed for the screening of gastric cancer to reduce its high mortality. We employed quantitative proteomics approach to develop discriminatory biomarker signatures from human saliva for the detection of gastric cancer. Salivary proteins were analyzed and compared between gastric cancer patients and matched control subjects by using tandem mass tags (TMT) technology. More than 500 proteins were identified with quantification, and 48 of them showed significant difference expression (p < 0.05) between normal controls and gastric cancer patients, including 7 up-regulated proteins and 41 down-regulated proteins. Five proteins were selected for initial verification by ELISA and three were successfully verified, namely cystatin B (CSTB), triosephosphate isomerase (TPI1), and deleted in malignant brain tumors 1 protein (DMBT1). All three proteins could differentiate gastric cancer patients from normal control subjects, dramatically (p < 0.05). The combination of these three biomarkers could reach 85% sensitivity and 80% specificity for the detection of gastric cancer with accuracy of 0.93. This study provides the proof of concept of salivary biomarkers for the non-invasive detection of gastric cancer. It is highly encouraging to turn these biomarkers into an applicable clinical test after large scale validation.

摘要

为降低胃癌的高死亡率，迫切需要新型生物标志物和非侵入性诊断方法用于胃癌筛查。我们采用定量蛋白质组学方法从人唾液中开发用于检测胃癌的鉴别生物标志物特征。通过串联质量标签（TMT）技术分析并比较了胃癌患者和匹配对照受试者的唾液蛋白质。定量鉴定出500多种蛋白质，其中48种在正常对照和胃癌患者之间表现出显著差异表达（p <0.05），包括7种上调蛋白和41种下调蛋白。选择5种蛋白质通过酶联免疫吸附测定（ELISA）进行初步验证，3种成功得到验证，即胱抑素B（CSTB）、磷酸丙糖异构酶（TPI1）和恶性脑肿瘤缺失1蛋白（DMBT1）。所有这三种蛋白质均可显著区分胃癌患者和正常对照受试者（p <0.05）。这三种生物标志物的组合对胃癌检测的敏感性可达85%，特异性可达80%，准确性为0.93。本研究为唾液生物标志物用于胃癌的非侵入性检测提供了概念验证。在大规模验证后将这些生物标志物转化为适用的临床试验，非常令人鼓舞。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/96f4f5219302/srep22165-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/45e91b47ac9a/srep22165-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/6b2d2dd6a01b/srep22165-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/0857dd53fee9/srep22165-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/43fd0acbb415/srep22165-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/1f3749b7798f/srep22165-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/ef0baf1691b6/srep22165-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/31f7b895424b/srep22165-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/96f4f5219302/srep22165-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/45e91b47ac9a/srep22165-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/6b2d2dd6a01b/srep22165-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/0857dd53fee9/srep22165-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/43fd0acbb415/srep22165-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/1f3749b7798f/srep22165-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/ef0baf1691b6/srep22165-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/31f7b895424b/srep22165-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7507/4766442/96f4f5219302/srep22165-f8.jpg

相似文献

1
Differential Proteomic Analysis of Human Saliva using Tandem Mass Tags Quantification for Gastric Cancer Detection.使用串联质谱标签定量技术对人唾液进行差异蛋白质组学分析以检测胃癌
Sci Rep. 2016 Feb 25;6:22165. doi: 10.1038/srep22165.
2
Evaluation of CSTB and DMBT1 expression in saliva of gastric cancer patients and controls.评估唾液中 CSTB 和 DMBT1 表达在胃癌患者和对照人群中的作用。
BMC Cancer. 2022 Apr 30;22(1):473. doi: 10.1186/s12885-022-09570-9.
3
Comparative proteomic analysis of human malignant ascitic fluids for the development of gastric cancer biomarkers.用于胃癌生物标志物开发的人恶性腹水的比较蛋白质组学分析
Clin Biochem. 2018 Jun;56:55-61. doi: 10.1016/j.clinbiochem.2018.04.003. Epub 2018 Apr 11.
4
Efficient isolation and proteomic analysis of cell plasma membrane proteins in gastric cancer reveal a novel differentiation and progression related cell surface marker, R-cadherin.胃癌细胞质膜蛋白的高效分离与蛋白质组学分析揭示了一种与分化和进展相关的新型细胞表面标志物——R-钙黏蛋白。
Tumour Biol. 2016 Sep;37(9):11775-11787. doi: 10.1007/s13277-016-5032-z. Epub 2016 Mar 30.
5
Serum Proteomic Analysis by Tandem Mass Tags (TMT) Based Quantitative Proteomics in Gastric Cancer Patients.基于串联质谱标签（TMT）的定量蛋白质组学对胃癌患者进行血清蛋白质组分析
Clin Lab. 2018 May 1;64(5):855-866. doi: 10.7754/Clin.Lab.2018.171129.
6
Discovery and preclinical validation of salivary transcriptomic and proteomic biomarkers for the non-invasive detection of breast cancer.唾液转录组学和蛋白质组学生物标志物用于非侵入性检测乳腺癌的发现和临床前验证。
PLoS One. 2010 Dec 31;5(12):e15573. doi: 10.1371/journal.pone.0015573.
7
Proteomic analysis of human saliva from lung cancer patients using two-dimensional difference gel electrophoresis and mass spectrometry.采用二维差异凝胶电泳和质谱技术对肺癌患者唾液进行蛋白质组学分析。
Mol Cell Proteomics. 2012 Feb;11(2):M111.012112. doi: 10.1074/mcp.M111.012112. Epub 2011 Nov 17.
8
Proteomics-Based Identification and Analysis of Proteins Associated with Helicobacter pylori in Gastric Cancer.基于蛋白质组学的胃癌中幽门螺杆菌相关蛋白质的鉴定与分析
PLoS One. 2016 Jan 8;11(1):e0146521. doi: 10.1371/journal.pone.0146521. eCollection 2016.
9
Identification and validation of salivary proteomic signatures for non-invasive detection of ovarian cancer.唾液蛋白质组学特征的鉴定和验证，用于卵巢癌的非侵入性检测。
Int J Biol Macromol. 2018 Mar;108:503-514. doi: 10.1016/j.ijbiomac.2017.12.014. Epub 2017 Dec 6.
10
Salivary proteome patterns of individuals exposed to High Altitude.高海拔暴露人群的唾液蛋白质组图谱。
Arch Oral Biol. 2018 Dec;96:104-112. doi: 10.1016/j.archoralbio.2018.09.002. Epub 2018 Sep 7.

引用本文的文献

1
Natural and induced immune responses in oral cavity and saliva.口腔和唾液中的天然免疫反应与诱导免疫反应。
BMC Immunol. 2025 Apr 18;26(1):34. doi: 10.1186/s12865-025-00713-8.
2
Saliva as a Diagnostic Tool for Systemic Diseases-A Narrative Review.唾液作为全身性疾病的诊断工具——一篇叙述性综述。
Medicina (Kaunas). 2025 Jan 30;61(2):243. doi: 10.3390/medicina61020243.
3
Relevance of Saliva Analyses in Terms of Etiological Factors, Biomarkers, and Indicators of Disease Course in Patients with Multiple Sclerosis-A Review.唾液分析在多发性硬化症患者病因学因素、生物标志物及疾病进程指标方面的相关性——综述

本文引用的文献

1
Tumour exosome integrins determine organotropic metastasis.肿瘤外泌体整合素决定器官特异性转移。
Nature. 2015 Nov 19;527(7578):329-35. doi: 10.1038/nature15756. Epub 2015 Oct 28.
2
Identification of the long non-coding RNA H19 in plasma as a novel biomarker for diagnosis of gastric cancer.血浆中长链非编码RNA H19作为胃癌诊断新生物标志物的鉴定。
Sci Rep. 2015 Jun 22;5:11516. doi: 10.1038/srep11516.
3
Update on metastatic gastric and esophageal cancers.转移性胃癌和食管癌的最新进展。
Int J Mol Sci. 2024 Nov 22;25(23):12559. doi: 10.3390/ijms252312559.
4
Salivary biomarkers for the diagnosis of gastric cancer: a systematic review.用于胃癌诊断的唾液生物标志物：一项系统综述。
Rev Cient Odontol (Lima). 2024 Jun 27;12(2):e199. doi: 10.21142/2523-2754-1202-2024-199. eCollection 2024 Apr-Jun.
5
Salivary metabolomic biomarkers for esophageal and gastric cancers by liquid chromatography-mass spectrometry.基于液相色谱-质谱联用的唾液代谢组学生物标志物用于食管和胃癌的诊断
Cancer Sci. 2024 Sep;115(9):3089-3098. doi: 10.1111/cas.16256. Epub 2024 Jul 14.
6
Liquid Biopsy: An Emerging Diagnostic, Prognostic, and Predictive Tool in Gastric Cancer.液体活检：胃癌中一种新兴的诊断、预后和预测工具。
J Gastric Cancer. 2024 Jan;24(1):4-28. doi: 10.5230/jgc.2024.24.e5.
7
Circulating Proteins as Diagnostic Markers in Gastric Cancer.循环蛋白作为胃癌的诊断标志物。
Int J Mol Sci. 2023 Nov 29;24(23):16931. doi: 10.3390/ijms242316931.
8
Telacebec Interferes with Virulence Lipid Biosynthesis Protein Expression and Sensitizes to Other Antibiotics.替拉塞贝克干扰毒力脂质生物合成蛋白表达并增强对其他抗生素的敏感性。
Microorganisms. 2023 Sep 30;11(10):2469. doi: 10.3390/microorganisms11102469.
9
Role of salivary miRNAs in the diagnosis of gastrointestinal disorders: a mini-review of available evidence.唾液微小RNA在胃肠道疾病诊断中的作用：现有证据的简要综述
Front Genet. 2023 Jun 29;14:1228482. doi: 10.3389/fgene.2023.1228482. eCollection 2023.
10
The salivary proteome in relation to oral mucositis in autologous hematopoietic stem cell transplantation recipients: a labelled and label-free proteomics approach.与自体造血干细胞移植受者口腔黏膜炎相关的唾液蛋白质组：一种标记和非标记蛋白质组学方法。
BMC Oral Health. 2023 Jul 7;23(1):460. doi: 10.1186/s12903-023-03190-w.
J Clin Oncol. 2015 Jun 1;33(16):1760-9. doi: 10.1200/JCO.2014.60.1799. Epub 2015 Apr 27.
4
Cancer statistics, 2015.癌症统计数据，2015 年。
CA Cancer J Clin. 2015 Jan-Feb;65(1):5-29. doi: 10.3322/caac.21254. Epub 2015 Jan 5.
5
Microfluidic optoelectronic sensor for salivary diagnostics of stomach cancer.用于胃癌唾液诊断的微流控光电传感器。
Biosens Bioelectron. 2015 May 15;67:465-71. doi: 10.1016/j.bios.2014.09.006. Epub 2014 Sep 6.
6
Genetics of gastric cancer.胃癌的遗传学。
Nat Rev Gastroenterol Hepatol. 2014 Nov;11(11):664-74. doi: 10.1038/nrgastro.2014.143. Epub 2014 Aug 19.
7
Global burden of gastric cancer attributable to Helicobacter pylori.幽门螺杆菌所致胃癌的全球负担。
Int J Cancer. 2015 Jan 15;136(2):487-90. doi: 10.1002/ijc.28999. Epub 2014 Jun 11.
8
Systematic review: serum autoantibodies in the early detection of gastric cancer.系统评价：血清自身抗体在胃癌早期检测中的应用
Int J Cancer. 2015 May 15;136(10):2243-52. doi: 10.1002/ijc.28807. Epub 2014 Mar 6.
9
Ion coalescence of neutron encoded TMT 10-plex reporter ions.中子编码 TMT 10 重试剂离子的离子聚结。
Anal Chem. 2014 Apr 1;86(7):3594-601. doi: 10.1021/ac500140s. Epub 2014 Mar 11.
10
Role of pancreatic cancer-derived exosomes in salivary biomarker development.胰腺癌衍生外泌体在唾液生物标志物开发中的作用。
J Biol Chem. 2013 Sep 13;288(37):26888-97. doi: 10.1074/jbc.M113.452458. Epub 2013 Jul 23.