Roa R C, Bose S K
Proc Natl Acad Sci U S A. 1975 Nov;72(11):4337-40. doi: 10.1073/pnas.72.11.4337.
Cultures of mouse Balb 3T3 fibroblasts exposed to a noncytotoxic dose of ethidium bromide for 16-18 hr are unable to produce foci after infection with murine sarcoma virus. Such cultures regain susceptibility to infection when incubated for 6-8 hr in drug-free growth medium. Pretreated but not untreated cultures exhibit sensitivity toward brief (6 hr) exposure to cycloheximide, chloramphenicol, and actinomycin D before infection. Pretreatment with cordy-cepin inhibits the ability of cultures to produce foci after infection. The recovery of ethidium-bromide-treated cultures requires the synthesis of cellular proteins which may have some important role in the establishment of RNA tumor virus infection.
用非细胞毒性剂量的溴化乙锭处理16 - 18小时的小鼠Balb 3T3成纤维细胞培养物，在感染鼠肉瘤病毒后无法形成病灶。当在无药物的生长培养基中孵育6 - 8小时时，此类培养物恢复对感染的敏感性。在感染前，经过预处理而非未处理的培养物对短暂（6小时）暴露于环己酰亚胺、氯霉素和放线菌素D表现出敏感性。用虫草素预处理会抑制培养物在感染后产生病灶的能力。溴化乙锭处理过的培养物的恢复需要合成细胞蛋白质，这些蛋白质可能在RNA肿瘤病毒感染的建立中具有一些重要作用。
