Bose S K, Zlotnick B J
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2374-8. doi: 10.1073/pnas.70.8.2374.
The earliest measurable parameter that shows density-dependent inhibition is the uptake of [(3)H]deoxyglucose by Balb 3T3 cells. The rate decreases even during the exponential phase of growth and reaches a minimum, about 8- to 10-times lower than maximum, as the culture approaches the saturation density. Cells transformed by murine sarcoma virus fail to show either growth-dependent or density-dependent inhibition of deoxyglucose uptake. Treatment with dibutyryl cyclic AMP in the presence of theophylline results in premature cessation of growth and in an arrest in the decline of deoxyglucose transport. Culture in serum-deficient medium also produces rapid inhibition of growth at low cell density, but these cultures exhibit a markedly decreased rate of deoxyglucose uptake.
最早可测量的显示密度依赖性抑制的参数是[(3)H]脱氧葡萄糖被Balb 3T3细胞摄取的情况。即使在生长的指数期，摄取速率也会下降，当培养物接近饱和密度时，摄取速率达到最小值，比最大值低约8至10倍。被鼠肉瘤病毒转化的细胞未能显示出对脱氧葡萄糖摄取的生长依赖性或密度依赖性抑制。在茶碱存在的情况下用二丁酰环磷腺苷处理会导致生长过早停止，并使脱氧葡萄糖转运的下降停滞。在低细胞密度下于血清缺乏的培养基中培养也会迅速抑制生长，但这些培养物中脱氧葡萄糖摄取的速率明显降低。
