猿猴病毒40 T抗原与DNA结合。
Simian virus 40 T antigen binds to DNA.
作者信息
Carroll R B, Hager L, Dulbecco R
出版信息
Proc Natl Acad Sci U S A. 1974 Sep;71(9):3754-7. doi: 10.1073/pnas.71.9.3754.
Abstract
The T antigen of simian virus 40, which may play a role in the control of viral DNA replication, is recovered from nuclei of cells transformed by simian virus 40 in several forms sedimenting at different rates. The large molecular weight forms are converted to the smallest (5 S) form by high salt, suggesting that they differ in the degree of aggregation. All the forms of the antigen bind efficiently to double-stranded DNA-cellulose columns at pH 6.2 and low salt, and elute in two fractions: one at pH 8.0 and low salt, the other at pH 8.0 and high salt. The antigen has little affinity for single-stranded DNA.
摘要
猴病毒40（Simian virus 40）的T抗原可能在病毒DNA复制的控制中发挥作用，它以几种沉降速率不同的形式从被猴病毒40转化的细胞核中分离出来。高分子量形式在高盐条件下会转化为最小的（5S）形式，这表明它们在聚集程度上存在差异。在pH 6.2和低盐条件下，该抗原的所有形式都能有效地结合到双链DNA纤维素柱上，并在两个组分中洗脱：一个在pH 8.0和低盐条件下，另一个在pH 8.0和高盐条件下。该抗原对单链DNA几乎没有亲和力。
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本文引用的文献
1
THE NATURE AND LOCALIZATION OF THE SV 40-INDUCED COMPLEMENT-FIXING ANTIGEN.猴空泡病毒40诱导的补体结合抗原的性质与定位
Proc Natl Acad Sci U S A. 1965 Mar;53(3):684-92. doi: 10.1073/pnas.53.3.684.
2
CELL-TRANSFORMING ABILITY OF A TEMPERATURE-SENSITIVE MUTANT OF POLYOMA VIRUS.多瘤病毒温度敏感突变体的细胞转化能力
Proc Natl Acad Sci U S A. 1965 Mar;53(3):486-91. doi: 10.1073/pnas.53.3.486.
3
DIFFERENTIAL EFFECTS OF INHIBITORS ON THE STEPS LEADING TO THE FORMATION OF SV40 TUMOR AND VIRUS ANTIGENS.抑制剂对导致SV40肿瘤和病毒抗原形成的各个步骤的不同作用。
J Exp Med. 1965 Jun 1;121(6):935-44. doi: 10.1084/jem.121.6.935.
4
SYNTHESIS OF SV40 TUMOR ANTIGEN DURING REPLICATION OF SIMIAN PAPOVAVIRUS (SV40).猴乳头瘤空泡病毒（SV40）复制过程中SV40肿瘤抗原的合成
Proc Natl Acad Sci U S A. 1964 Nov;52(5):1138-42. doi: 10.1073/pnas.52.5.1138.
5
A SPECIFIC COMPLEMENT-FIXING ANTIGEN PRESENT IN SV40 TUMOR AND TRANSFORMED CELLS.SV40肿瘤细胞和转化细胞中存在的一种特异性补体结合抗原。
Proc Natl Acad Sci U S A. 1963 Dec;50(6):1148-56. doi: 10.1073/pnas.50.6.1148.
6
7
Studies with DNA-cellulose chromatography. I. DNA-binding proteins from Escherichia coli.DNA-纤维素色谱法研究。I. 来自大肠杆菌的DNA结合蛋白。
Cold Spring Harb Symp Quant Biol. 1968;33:289-305. doi: 10.1101/sqb.1968.033.01.033.
8
Studies of the binding of Escherichia coli RNA polymerase to DNA. I. The role of sigma subunit in site selection.大肠杆菌RNA聚合酶与DNA结合的研究。I. σ亚基在位点选择中的作用。
J Mol Biol. 1972 Sep 28;70(2):157-85. doi: 10.1016/0022-2836(72)90531-1.
9
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Virology. 1973 Sep;55(1):127-35. doi: 10.1016/s0042-6822(73)81014-1.
10
The transformation of BHK 21 hamster cells by simian virus 40.猿猴病毒40对BHK 21仓鼠细胞的转化
Int J Cancer. 1972 Sep 15;10(2):296-309. doi: 10.1002/ijc.2910100210.
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