[饮用水消毒副产物氯乙酸诱导大鼠p53基因DNA损伤的研究]
[Study on rat's DNA damage of p53 induced by chlorinated acetic acids of drinking water disinfection by-products].
作者信息
Yang Yuan, Heng Zheng-chang
机构信息
West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Wei Sheng Yan Jiu. 2006 Nov;35(6):683-5.
OBJECTIVE
To study on the DNA damage of p53 induced by dichloroacetic acid(DCA) and trichloroacetic acid( TCA), approve their genotoxicity and discuss molecular mechanism of their carcinogenic action.
METHODS
Administered SD rats with DCA or TCA by i.p. injection, extracted DNA from rat's liver, and then used RDPCR to detect DNA damage of exon 70f p53 gene.
RESULTS
Two hybridization bands were detected in treated group induced by DCA. It was indicated that DCA can result in DNA damage of exon 7 of p53 gene of rat's liver tissue, and there were two broken sites. It was not detected damage of exon 7 of p53 gene of rat induced by TCA.
CONCLUSION
There may be the relationship between DCA carcinogenic action and the damage of p53. The result that the damage of p53 of target tissue detected by RDPCR was consistent well with rat carcinogenic test.
目的
研究二氯乙酸（DCA）和三氯乙酸（TCA）诱导的p53基因DNA损伤，证实其遗传毒性并探讨其致癌作用的分子机制。
方法
通过腹腔注射给予SD大鼠DCA或TCA，从大鼠肝脏中提取DNA，然后用RDPCR检测p53基因第7外显子的DNA损伤。
结果
DCA处理组检测到两条杂交带。表明DCA可导致大鼠肝脏组织p53基因第7外显子的DNA损伤，且有两个断裂位点。未检测到TCA诱导的大鼠p53基因第7外显子损伤。
结论
DCA致癌作用可能与p53损伤有关。RDPCR检测靶组织p53损伤的结果与大鼠致癌试验结果吻合良好。
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