[联苯胺诱导大鼠p53基因损伤及器官特异性的研究]
[Study of rat's p53 gene damage and organ specificity induced by benzidine].
作者信息
Wu Qing, Heng Zheng-chang
机构信息
Department of Environmental Health, West China School of Public Health, Sichuan University, Chengdu 610041, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2006 Jan;37(1):33-4, 39.
OBJECTIVE
Studying the main target organs and the genetic toxicity mechanism of benzidine.
METHODS
SD rats were given benzidine i.p. injection. DNA was extracted from rat's liver, kidney, lung and bladders. Then ss probes RDPCR was used to detect the damaged DNA of exon 7 of p53 gene.
RESULTS
Hybridization bands were found in liver, bladder and lung tissues after ss probes RDPCR, while no hybridization bands were found in kidney tissues.
CONCLUSION
The result indicates that benzidine can cause the DNA lesions of exon 7 of p53 gene and its major target organs are liver, bladder and lung. The toxicity mechanism of benzidine is probably related to p53 gene damage.
目的
研究联苯胺的主要靶器官及遗传毒性机制。
方法
对SD大鼠进行腹腔注射联苯胺。从大鼠的肝脏、肾脏、肺和膀胱中提取DNA。然后使用单链探针RDPCR检测p53基因第7外显子的受损DNA。
结果
单链探针RDPCR检测后，在肝脏、膀胱和肺组织中发现杂交带，而在肾脏组织中未发现杂交带。
结论
结果表明，联苯胺可导致p53基因第7外显子的DNA损伤，其主要靶器官为肝脏、膀胱和肺。联苯胺的毒性机制可能与p53基因损伤有关。
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