Suppr超能文献

肥大细胞脱颗粒在哮喘小鼠模型即刻过敏反应神经关联中的作用

Role of mast cell degranulation in the neural correlates of the immediate allergic reaction in a murine model of asthma.

作者信息

Costa-Pinto Frederico Azevedo, Basso Alexandre Salgado, Russo Momtchilo

机构信息

Department of Pathology, School of Veterinary Medicine, University of Sao Paulo, Brazil.

出版信息

Brain Behav Immun. 2007 Aug;21(6):783-90. doi: 10.1016/j.bbi.2007.01.002. Epub 2007 Feb 8.

Abstract

Experimental airway allergy in mice leads to increased activity in specific hypothalamic and amygdaloid nuclei, and behavioral changes. The experiments described here were designed to determine the role of anaphylactic antibodies, mast cell degranulation, and lung inflammation in the neural and behavioral correlates of an experimental murine asthma-like response. Animals were sensitized intraperitoneally with ovalbumin adsorbed to alum, and challenged by intranasal ovalbumin instillation or aerosol. To induce immunological tolerance, animals were fed ovalbumin in the drinking water for 5 consecutive days, along with primary sensitization. Depletion of IgE was also accomplished with a non-anaphylactic anti-IgE antibody. Mast cell degranulation was inhibited by cromolyn. In addition to BALB/c animals, C3H/HeJ mice were used for their relative resistance to lung allergic inflammation. We confirmed that ovalbumin challenge in allergic mice leads to increased activity in the paraventricular nucleus of the hypothalamus and central nucleus of the amygdala, and avoidance behavior towards an allergen-associated compartment. Moreover, these responses were precluded by oral tolerance or anti-IgE treatment, even in the presence of IgG1. Cromolyn abrogates both responses in the presence of anaphylactic antibodies. Finally, although sensitized C3H/HeJ mice did not develop airway inflammation, they exhibited brain and behavioral changes similar to BALB/c animals. The repercussions of murine allergic asthma on brain and behavior are IgE-dependent, mediated by mast cell degranulation, and do not require a pulmonary inflammatory infiltrate, suggesting that the early phase of this immediate allergic response suffices for the brain activation associated with avoidance behavior towards exposure to the allergen.

摘要

小鼠实验性气道过敏会导致特定下丘脑和杏仁核核团活动增加以及行为改变。本文所述实验旨在确定过敏抗体、肥大细胞脱颗粒和肺部炎症在实验性小鼠哮喘样反应的神经及行为关联中的作用。动物通过腹腔注射吸附于明矾的卵清蛋白进行致敏,并通过鼻内滴注或雾化卵清蛋白进行激发。为诱导免疫耐受,在初次致敏的同时,让动物连续5天饮用含卵清蛋白的水。还用非过敏性抗IgE抗体实现了IgE的耗竭。色甘酸抑制肥大细胞脱颗粒。除BALB/c动物外,还使用了C3H/HeJ小鼠,因为它们对肺部过敏性炎症具有相对抗性。我们证实,对过敏性小鼠进行卵清蛋白激发会导致下丘脑室旁核和杏仁核中央核的活动增加,以及对过敏原相关隔室的回避行为。此外,即使存在IgG1,口服耐受或抗IgE治疗也能消除这些反应。在存在过敏抗体的情况下,色甘酸可消除这两种反应。最后,尽管致敏的C3H/HeJ小鼠未发生气道炎症,但它们表现出与BALB/c动物相似的脑和行为变化。小鼠过敏性哮喘对脑和行为的影响是IgE依赖性的,由肥大细胞脱颗粒介导,且不需要肺部炎性浸润,这表明这种速发型过敏反应的早期阶段足以激活与回避接触过敏原相关的脑区。

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验