Valli Alessandro, Suliman Mohamed E, Meert Natalie, Vanholder Raymond, Lindholm Bengt, Stenvinkel Peter, Watanabe Makoto, Barany Peter, Alvestrand Anders, Anderstam Björn
Divisions of Renal Medicine and Baxter Novum, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Huddinge, Stockholm, Sweden.
Clin Chim Acta. 2007 Apr;379(1-2):87-94. doi: 10.1016/j.cca.2006.12.026. Epub 2007 Jan 16.
Advanced oxidation protein products (AOPP), a suggested protein biomarker of oxidative stress, are elevated in patients with chronic kidney disease (CKD), who also often suffer from hypertriglyceridemia.
The analysis included plasma AOPP, TG, cholesterol, albumin and total protein, inflammation and oxidative stress markers from healthy subjects, non-dialyzed CKD, HD and CAPD patients. We studied, at two different European centres, effects of a meal, comparison between serum and plasma, L-index (indicating turbidity), spiking with fat and protein, and centrifugation on the AOPP concentrations. AOPP was measured at 340 nm and expressed as chloramine-T equivalents.
AOPP correlated with TG levels not only in CKD patients, but also in healthy subjects. Weak to absent correlations were observed between AOPP and markers of inflammation and oxidative stress in CKD patients. A meal increased the TG levels several-fold paralleled by a rise in measured AOPP to patient levels. Spiking of the plasma with Intralipid or protein resulted in increased absorbances at 340 nm, due to turbidity or real absorbance, while centrifugation similarly decreased the apparent AOPP and TG levels.
AOPP concentration, especially due to the influence of turbidity at all levels of TG concentration, but also due to other factors on top of TG, is overestimated in all plasma samples, including controls at fasting and non-fasting conditions. Thus, AOPP is a questionable biomarker of oxidative stress and inflammation in CKD patients.
晚期氧化蛋白产物（AOPP）是一种氧化应激的蛋白质生物标志物，在慢性肾脏病（CKD）患者中升高，这些患者也常患有高甘油三酯血症。
分析纳入了健康受试者、未透析的CKD患者、血液透析（HD）患者和持续性不卧床腹膜透析（CAPD）患者的血浆AOPP、甘油三酯（TG）、胆固醇、白蛋白和总蛋白、炎症及氧化应激标志物。我们在两个不同的欧洲中心研究了进餐的影响、血清与血浆的比较、L指数（表示浊度）、脂肪和蛋白质加样以及离心对AOPP浓度的影响。AOPP在340nm处测量，并以氯胺 - T当量表示。
AOPP不仅与CKD患者的TG水平相关，也与健康受试者的TG水平相关。在CKD患者中，AOPP与炎症和氧化应激标志物之间观察到弱相关性或无相关性。进餐使TG水平升高数倍，同时测得的AOPP升高至患者水平。用英脱利匹特或蛋白质对血浆进行加样，由于浊度或实际吸光度，导致在340nm处吸光度增加，而离心同样降低了表观AOPP和TG水平。
在所有血浆样本中，包括空腹和非空腹状态下的对照样本，AOPP浓度都被高估了，这尤其是由于在所有TG浓度水平下浊度的影响，但也由于TG之外的其他因素。因此，AOPP作为CKD患者氧化应激和炎症的生物标志物存在疑问。
