Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Murcia, Spain.
BMC Vet Res. 2021 Jun 26;17(1):226. doi: 10.1186/s12917-021-02924-8.
Reactive oxygen species (ROS) are reactive compounds derived from oxygen. In biological systems, an excessive amount of ROS can cause oxidative damage to biological macromolecules being involved in different diseases. Several assays have been developed in the last 30 years for ROS evaluation. The objective of this article will be to provide an update about the spectrophotometric methods currently used in the assessment of ROS in serum. The chemical basis of four different techniques will be reviewed, and examples of their possible applications will be provided. A particular emphasis about the practical applications of these assays in the dog will be made, but selected information about their use in humans will also be presented for comparative purposes, following a One-Health approach. The information about the spectrophotometric assays presented in this paper should be interpreted with caution once limited information about them is available yet, and further studies should be performed to clarify what they measure and their clinical application. Ideally, when applied to evaluate a sample's oxidative status, they should be incorporated in a panel of analytes where other oxidants, antioxidants, and biomarkers of inflammation were also included.
活性氧（ROS）是源自氧的反应性化合物。在生物系统中，过量的 ROS 会导致生物大分子发生氧化损伤，从而涉及到不同的疾病。在过去的 30 年中，已经开发了几种用于评估 ROS 的测定方法。本文的目的将提供关于目前用于评估血清中 ROS 的分光光度法的最新信息。将回顾四种不同技术的化学基础，并提供它们可能应用的示例。将特别强调这些测定在犬中的实际应用，但也将根据“同一健康”方法，提供关于它们在人类中应用的相关信息。由于目前关于这些测定方法的信息有限，因此应谨慎解释本文中介绍的分光光度测定方法的信息，并且应进行进一步的研究以阐明它们的测量内容及其临床应用。理想情况下，当应用于评估样品的氧化状态时，它们应该包含在一组分析物中，其中还包括其他氧化剂、抗氧化剂和炎症生物标志物。
