Sommerhalder Rubik J, McDonald Bruce A, Zhan Jiasui
Plant Pathology, Institute of Integrative Biology, ETH Zurich, LFW, Universitaetstrasse 2, CH-8092 Zürich, Switzerland.
Fungal Genet Biol. 2007 Aug;44(8):764-72. doi: 10.1016/j.fgb.2007.01.003. Epub 2007 Jan 10.
We compared patterns of mitochondrial restriction fragment length polymorphism (RFLP) diversity with patterns of nuclear RFLP diversity to investigate the effects of selection, gene flow, and sexual reproduction on the population genetic structure and evolutionary history of the wheat pathogen Phaeosphaeria nodorum. A total of 315 fungal isolates from Texas, Oregon, and Switzerland were analyzed using seven nuclear RFLP probes that hybridized to discrete loci and purified mitochondrial DNA that hybridized to the entire mtDNA genome. Forty-two different mitochondrial haplotypes and 298 different nuclear haplotypes were detected. The two most frequent mtDNA haplotypes were present in every population and represented 32% of all isolates. High levels of gene flow, low levels of population subdivision, no evidence for either host specificity or cyto-nuclear disequilibrium were inferred from the analysis of both genomes. The concordance in estimates of these population genetic parameters from both genomes suggests that the two genomes experienced similar degrees of migration, genetic drift and selection.
我们比较了线粒体限制性片段长度多态性（RFLP）多样性模式与核RFLP多样性模式，以研究选择、基因流和有性繁殖对小麦病原菌小麦叶枯病菌（Phaeosphaeria nodorum）种群遗传结构和进化历史的影响。使用7个与离散位点杂交的核RFLP探针以及与整个线粒体DNA基因组杂交的纯化线粒体DNA，对来自得克萨斯州、俄勒冈州和瑞士的总共315个真菌分离株进行了分析。检测到42种不同的线粒体单倍型和298种不同的核单倍型。两种最常见的线粒体DNA单倍型存在于每个种群中，占所有分离株的32%。对两个基因组的分析推断出高水平的基因流、低水平的种群细分，没有宿主特异性或细胞质-核不平衡的证据。两个基因组对这些种群遗传参数估计的一致性表明，两个基因组经历了相似程度的迁移、遗传漂变和选择。
