Degradi Luca, Tava Valeria, Prigitano Anna, Esposto Maria Carmela, Tortorano Anna Maria, Saracchi Marco, Kunova Andrea, Cortesi Paolo, Pasquali Matias
Department of Food, Environmental and Nutritional Science (DeFENS), University of Milan, Via Celoria 2, 20133 Milan, Italy.
Department of Biomedical Sciences for Health, University of Milan, Via Pascal 36, 20133 Milan, Italy.
Microorganisms. 2022 May 28;10(6):1115. doi: 10.3390/microorganisms10061115.
has recently been described as a cross-kingdom pathogen causing post-harvest disease in bananas and systemic and superficial infection in humans. The taxonomic identity of fungal cross-kingdom pathogens is essential for confirming the identification of the species on distant infected hosts. Understanding the level of variability within the species is essential to decipher the population homogeneity infecting human and plant hosts. In order to verify that strains isolated from fruits and patients are part of a common population and to estimate their overall diversity, we assembled, annotated and explored the diversity of the mitogenomes of 18 strains obtained from banana fruits and human patients. The mitogenomes showed a high level of similarity among strains with different hosts' origins, with sizes ranging from 56,493 to 59,256 bp. All contained 27 tRNA genes and 14 protein-coding genes, rps3 protein, and small and large ribosomal subunits (rns and rnl). Variations in the number of endonucleases were detected. A comparison of mitochondrial endonucleases distribution with a diverse set of mitogenomes allowed us to specifically discriminate from its sister species and the other species. Despite the diversity in mitochondria, strains from bananas and strains from human patients group together, indirectly confirming as a cross-kingdom pathogen.
最近，它被描述为一种跨界病原体，可导致香蕉采后病害以及人类的全身和浅表感染。真菌跨界病原体的分类身份对于确认在远距离感染宿主上的物种鉴定至关重要。了解物种内的变异水平对于解读感染人类和植物宿主的种群同质性至关重要。为了验证从水果和患者中分离出的菌株是否属于一个共同种群，并估计它们的总体多样性，我们组装、注释并探索了从香蕉果实和人类患者中获得的18株菌株的有丝分裂基因组的多样性。这些有丝分裂基因组在不同宿主来源的菌株之间显示出高度相似性，大小范围从56,493到59,256 bp。所有基因组都包含27个tRNA基因、14个蛋白质编码基因、rps3蛋白以及小和大核糖体亚基（rns和rnl）。检测到核酸内切酶数量的变化。通过将线粒体核酸内切酶分布与一组多样的有丝分裂基因组进行比较，我们能够从其姊妹物种和其他物种中特异性地区分该物种。尽管该物种的线粒体存在多样性，但来自香蕉的菌株和来自人类患者的菌株聚集在一起，间接证实了该物种是一种跨界病原体。
