假性剥脱综合征患者房水和血清中的蛋白质羰基水平。
Protein carbonyl levels in the aqueous humour and serum of patients with pseudoexfoliation syndrome.
作者信息
Yagci R, Ersöz I, Erdurmuş M, Gürel A, Duman S
机构信息
Department of Ophthalmology, Medical School, Fatih University, Ankara, Turkey.
出版信息
Eye (Lond). 2008 Jan;22(1):128-31. doi: 10.1038/sj.eye.6702751. Epub 2007 Feb 9.
PURPOSE
Protein oxidation is an oxidative stress marker and the oxidation of proteins is analysed by measuring the carbonyl groups. Protein oxidation can have a role in the physiopathology of pseudoexfoliation (PEX) syndrome. The aim of this study was to investigate the protein oxidation in the aqueous humour and serum of cataract patients with and without PEX.
METHODS
A multicenter study was carried out. Aqueous humour and serum samples were collected from patients who underwent routine cataract surgery. Patients were divided into PEX (n=29) and control (n=27) groups. Patients had no elevated intraocular pressure or glaucoma. Spectrophotometer was used to measure protein carbonyl (PC) levels in the samples.
RESULTS
Mean PC concentration in the PEX aqueous (2.18+/-1.51 nmol/l) and serum (119.62+/-13.2 nmol/l) samples was significantly higher than that measured in the control aqueous (1.31+/-0.47 nmol/l) and serum (105.85+/-11.76 nmol/l) samples, respectively (P< 0.001).
CONCLUSION
The increased PC levels in the aqueous humour and serum of PEX patients suggest that protein oxidation may play a role in the physiopathology of PEX.
目的
蛋白质氧化是一种氧化应激标志物，可通过测量羰基来分析蛋白质的氧化情况。蛋白质氧化可能在假性剥脱（PEX）综合征的生理病理学中起作用。本研究的目的是调查患有和未患有PEX的白内障患者房水和血清中的蛋白质氧化情况。
方法
开展了一项多中心研究。从接受常规白内障手术的患者中收集房水和血清样本。患者被分为PEX组（n = 29）和对照组（n = 27）。患者眼压未升高且无青光眼。使用分光光度计测量样本中的蛋白质羰基（PC）水平。
结果
PEX组房水（2.18±1.51 nmol/l）和血清（119.62±13.2 nmol/l）样本中的平均PC浓度分别显著高于对照组房水（1.31±0.47 nmol/l）和血清（105.85±11.76 nmol/l）样本中的浓度（P < 0.001）。
结论
PEX患者房水和血清中PC水平升高表明蛋白质氧化可能在PEX的生理病理学中起作用。
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