Berlau J, Lorenz P, Beck R, Makovitzky J, Schlötzer-Schrehardt U, Thiesen H J, Guthoff R
Department of Ophthalmology, University of Rostock, Germany.
Graefes Arch Clin Exp Ophthalmol. 2001 Oct;239(10):743-6. doi: 10.1007/s004170100357.
Pseudoexfoliation syndrome (PEX) has been suggested to represent a blood-aqueous barrier impairment leading to a higher protein content in aqueous humour of eyes with PEX. However, the nature of a prospective PEX protein has not yet been described. We set out to reevaluate protein content and examine protein composition for prospective PEX protein candidates in aqueous humour of eyes with PEX syndrome. Aqueous humour of 52 patients with PEX and 38 without PEX signs was sampled during cataract or glaucoma surgery. Total aqueous protein concentration in the samples was analysed in 43 PEX specimens and 32 non-PEX specimens according to Bradford. Aqueous protein composition of all samples was determined by sodium dodecylsulphate polyacrylamide gel electrophoresis (SDS PAGE) and silver staining. Screening for amyloids was performed in nine PEX samples and six non-PEX samples by Congo Red staining and polarised light microscopy. Aqueous protein concentration was not significantly increased in PEX eyes in comparison with non-PEX eyes. Furthermore, we could not detect any characteristic difference in protein band sizes of the two groups after SDS PAGE. However, we were able to show the presence of amyloid exclusively in aqueous humour of PEX patients.
our results do not confirm a generally higher protein concentration in pseudoexfoliation syndrome eyes. This does not necessarily contradict a blood-aqueous barrier impairment but illustrates the variance in protein concentration between and within the two groups. No characteristic protein band allocatable to pseudoexfoliation syndrome proteins could be detected in any of the samples. However, our findings support the theory that the pseudoexfoliation syndrome is associated with an amyloid of a serum protein.
有人提出假性剥脱综合征（PEX）代表血-房水屏障受损，导致患有PEX的眼睛房水中蛋白质含量升高。然而，尚未描述潜在的PEX蛋白的性质。我们着手重新评估蛋白质含量，并检查患有PEX综合征的眼睛房水中潜在的PEX蛋白候选物的蛋白质组成。在白内障或青光眼手术期间采集了52例患有PEX的患者和38例无PEX体征的患者的房水。根据Bradford法分析了43份PEX标本和32份非PEX标本中样品的总房水蛋白浓度。通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和银染法测定所有样品的房水蛋白组成。通过刚果红染色和偏振光显微镜对9份PEX样品和6份非PEX样品进行淀粉样蛋白筛查。与非PEX眼睛相比，PEX眼睛中的房水蛋白浓度没有显著增加。此外，SDS-PAGE后，我们未检测到两组蛋白质条带大小有任何特征性差异。然而，我们能够证明仅在PEX患者的房水中存在淀粉样蛋白。
我们的结果未证实假性剥脱综合征眼睛中蛋白质浓度普遍较高。这不一定与血-房水屏障受损相矛盾，但说明了两组之间以及组内蛋白质浓度的差异。在任何样品中均未检测到可归因于假性剥脱综合征蛋白的特征性蛋白条带。然而，我们的研究结果支持假性剥脱综合征与一种血清蛋白的淀粉样蛋白有关的理论。
