Zhang G Q, Ge X N, Guo X, Yang H C
Key Laboratory of Preventive Veterinary Medicine of Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing, P.R. China.
Arch Virol. 2007;152(6):1209-13. doi: 10.1007/s00705-006-0930-9. Epub 2007 Feb 9.
Two strains of encephalomyocarditis virus (EMCV), designated BJC3 and HB1, were isolated from an aborted fetus and the heart tissue of a dead piglet that had pericardial fluid, respectively. The complete genomic sequences of the two viruses were determined and analyzed. The size of the genomes of BJC3 and HB1 were 7746 and 7735 nucleotides, respectively, including poly(A) tails. Comparative analysis with the genomic sequences of other EMCV strains showed that BJC3 and HB1 shared higher identity (92.5-99.6%) with BEL-2887A/91, EMCV-R and PV21, but lower identity (83.3-84.6%) with EMC-B, EMC-D and D variants, and only 81.0% with Mengo virus. Two amino acid mutations in the leader protein of the two viruses and one amino acid substitution in VP1 of BJC3 were found in comparison to other EMCV strains Phylogenetic analysis based on the amino acid sequences of the entire ORF revealed that the two Chinese isolates BJC3 and HB1 clustered together with the strains BEL-2887/91, EMCV-R and PV21, which belong to the same genetic subgroup as EMCV-30. Our results provide genomic information for EMCV isolated in China.
从一头流产仔猪以及一头心包积液死亡仔猪的心脏组织中分别分离出两株脑心肌炎病毒（EMCV），命名为BJC3和HB1。测定并分析了这两种病毒的完整基因组序列。BJC3和HB1的基因组大小分别为7746和7735个核苷酸，包括poly(A)尾。与其他EMCV毒株的基因组序列进行比较分析表明，BJC3和HB1与BEL-2887A/91、EMCV-R和PV21的同源性较高（92.5-99.6%），但与EMC-B、EMC-D和D变种的同源性较低（83.3-84.6%），与Mengo病毒的同源性仅为81.0%。与其他EMCV毒株相比，发现这两种病毒的前导蛋白中有两个氨基酸突变，BJC3的VP1中有一个氨基酸替换。基于整个ORF氨基酸序列的系统发育分析表明，中国分离株BJC3和HB1与BEL-2887/91、EMCV-R和PV21毒株聚集在一起，它们与EMCV-30属于同一遗传亚组。我们的结果为在中国分离的EMCV提供了基因组信息。
