中国北方猪脑心肌炎病毒BD2株的分离与分子分析
Isolation and molecular analysis of porcine encephalomyocarditis virus strain BD2 from northern China.
作者信息
Yuan Wanzhe, Song Qinye, Zhang Xiuyuan, Zhang Lingling, Sun Jiguo
机构信息
College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
College of Animal Medicine, Agricultural University of Hebei, Baoding, Hebei 071001, China; Hebei Engineering and Technology Research Center of Veterinary Biological Products, Baoding, Hebei 071001, China; North China Research Center of Animal Epidemic Pathogen Biology, China Agriculture Ministry, Baoding, Hebei 071001, China.
出版信息
Infect Genet Evol. 2014 Jan;21:303-7. doi: 10.1016/j.meegid.2013.11.014. Epub 2013 Nov 28.
Encephalomyocarditis virus (EMCV) can cause acute myocarditis in young pigs or reproductive failure in sows. In this study, an EMCV strain (BD2) was isolated from the suspected piglets with EMCV in China. It was identified by an indirect immunofluorescence assay and reverse-transcription polymerase chain reaction. The virus could reproduce on BHK-21 cells and reach a peak titer by 16 hpi with maximum titers of 10(8.22)TCID50/0.1 ml. Phylogenetic analyses of open reading frame and the VP3/VP1 genes using neighbor-joining method revealed that EMCV isolates fell into two clusters: groups I and II. The BD2 isolate belonged to group I, along with strains NJ08, HB1, BJC3, CBNU, K3, K11, BEL-2887A, GX0601, GXLC, pEC9, and PV21, whereas four strains (D variant, EMCV-B, EMCV-D, and PV2) belonged to group II.
脑心肌炎病毒（EMCV）可导致幼猪急性心肌炎或母猪繁殖障碍。在本研究中，从中国疑似感染EMCV的仔猪中分离出一株EMCV毒株（BD2）。通过间接免疫荧光试验和逆转录聚合酶链反应对其进行鉴定。该病毒可在BHK-21细胞上繁殖，在感染后16小时达到滴度峰值，最高滴度为10(8.22)TCID50/0.1 ml。使用邻接法对开放阅读框和VP3/VP1基因进行系统发育分析，结果显示EMCV分离株分为两个簇：I组和II组。BD2分离株与NJ08、HB1、BJC3、CBNU、K3、K11、BEL-2887A、GX0601、GXLC、pEC9和PV21毒株同属于I组，而四个毒株（D变异株、EMCV-B、EMCV-D和PV2）属于II组。
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