Shi Kai-Chuang, Qu Su-Jie, Chen Jin-Xi, Xu Rui-Sheng, Zheng Min, Liu Qi, Chen Han-Zhong, Li Gang
Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Bing Du Xue Bao. 2010 Mar;26(2):134-42.
The complete genome of encephalomyocarditis virus (EMCV)strain GXLC isolated from swine was sequenced and analyzed. Five overlapped gene fragments covering the entire open reading frame (ORF) were amplified by RT-PCR, and the 3'-untranslated region (UTR) and 5'-UTR were amplified by the 3'-rapid amplification of cDNA ends (RACE) and 5'-RACE method, respectively. The genome sequences of strain GXLC were obtained by assembling the sequences of RT-PCR-generated cDNA fragments. The length of the complete genome was 7 725 nucleotides (nt). The homology comparison and phylogenetic analysis of the nucleotide and deduced amino acid sequences between strain GXLC and other EMCV strains available in GenBank were performed. The results showed that the complete genome identity between GXLC strain and the strains from China, i.e. GX0601, GX0602, BJC3 and HB1 and the strains from other countries, i.e. CBNU, K3, K11, TEL-2887A, EMCV-R and PV21 was over 99%. The phylogenetic trees based on the complete genome, the structural protein or the non-structural protein gene sequences revealed that the tree topology was similar. All the EMCV strains could be divided into two groups: group I and group II, and group I could be subdivided into subgroup Ia and subgroup Ib. The strains from swine belonged to subgroup Ia or Ib, and the strains from mice belonged to subgroup Ia, while the strains from Sus scro fa belonged to group II. Strain GXLC, together with other EMCV isolates from China, belonged to subgroup Ia.
对从猪中分离出的脑心肌炎病毒（EMCV）GXLC株的全基因组进行了测序和分析。通过RT-PCR扩增了覆盖整个开放阅读框（ORF）的5个重叠基因片段，分别采用3'-cDNA末端快速扩增（RACE）和5'-RACE方法扩增3'-非翻译区（UTR）和5'-UTR。通过组装RT-PCR产生的cDNA片段序列获得了GXLC株的基因组序列。完整基因组长度为7725个核苷酸（nt）。对GXLC株与GenBank中其他EMCV株的核苷酸和推导氨基酸序列进行了同源性比较和系统发育分析。结果表明，GXLC株与来自中国的株系即GX0601、GX0602、BJC3和HB1以及来自其他国家的株系即CBNU、K3、K11、TEL-2887A、EMCV-R和PV21的全基因组同一性超过99%。基于全基因组、结构蛋白或非结构蛋白基因序列构建的系统发育树显示，树的拓扑结构相似。所有EMCV株可分为两组：I组和II组，I组可进一步细分为Ia亚组和Ib亚组。来自猪的株系属于Ia亚组或Ib亚组，来自小鼠的株系属于Ia亚组，而来自野猪的株系属于II组。GXLC株与来自中国的其他EMCV分离株一起属于Ia亚组。
