Nakama K, Gotoh M, Yamada T, Mitsui Y, Yasukawa H, Imaizumi T, Higuchi F, Nagata K
Department of Orthopaedic Surgery, Kurume University, Fukuoka, Japan.
J Int Med Res. 2006 Nov-Dec;34(6):624-31. doi: 10.1177/147323000603400607.
The aim of this study was to examine interleukin-6 production and the activation of signal transducer and activator of transcription-3 (STAT3) in ruptured rotator cuff tendon. Specimens of ruptured rotator cuff tendons were analysed using real-time reverse transcriptase polymerase chain reaction, Western blotting and immunohistochemistry. Specimens of co-existing inflammatory subacromial synovia were examined for comparison. The level of interleukin-6 messenger RNA was increased in ruptured rotator cuff tendon as well as in subacromial synovium. Western blot analysis showed constitutive production of activated, phosphorylated STAT3 in ruptured rotator cuff tendon and co-existing subacromial synovium. Immunohistochemical examination detected cells producing interleukin-6, interleukin-6 receptor and phosphorylated STAT3 in ruptured rotator cuff tendon, mainly in proliferative vessels and, to a lesser extent, in tendon fibroblasts around the vessels. This study demonstrates that activation of STAT3 induced by interleukin-6 is promoted mainly by proliferative vessels in ruptured rotator cuff tendon.
本研究的目的是检测肩袖肌腱断裂时白细胞介素-6的产生及信号转导和转录激活因子3（STAT3）的激活情况。采用实时逆转录聚合酶链反应、蛋白质免疫印迹法和免疫组织化学法对肩袖肌腱断裂标本进行分析。同时检测并存的炎性肩峰下滑膜标本以作比较。肩袖肌腱断裂处及肩峰下滑膜中白细胞介素-6信使核糖核酸水平均升高。蛋白质免疫印迹分析显示，在肩袖肌腱断裂处及并存的肩峰下滑膜中存在组成性产生的活化磷酸化STAT3。免疫组织化学检查在肩袖肌腱断裂处检测到产生白细胞介素-6、白细胞介素-6受体和磷酸化STAT3的细胞，主要存在于增生血管中，在血管周围的肌腱成纤维细胞中也有少量存在。本研究表明，白细胞介素-6诱导的STAT3激活主要由肩袖肌腱断裂处的增生血管促进。
