Pascal Thierry, Debacq-Chainiaux Florence, Boilan Emmanuelle, Ninane Noëlle, Raes Martine, Toussaint Olivier
Unit of Research on Cellular Biology (URBC), Department of Biology, University of Namur (FUNDP), Rue de Bruxelles, 5000, Namur, Belgium.
Biogerontology. 2007 Aug;8(4):409-22. doi: 10.1007/s10522-007-9084-8. Epub 2007 Feb 13.
Acute repeated exposures to subcytotoxic concentrations of tert-butylhydroperoxide and ethanol trigger premature senescence of human diploid fibroblasts. In the present work we found an increased mRNA and protein level of interleukin-11 and heme oxygenase-1 in premature senescence of WI-38 human diploid foetal lung fibroblasts induced by both tert-butylhydroperoxide and ethanol. We tested whether interleukin-11 and heme oxygenase-1 could protect against tert-butylhydroperoxide- or ethanol-induced premature senescence when stable overexpression was established using a retroviral vector-based transduction. No protective effect was found against the decrease of the proliferative potential, the increase of the proportion of senescence-associated ss-galactosidase positive cells and the increase of the mRNA levels of six senescence-associated genes.
急性反复暴露于亚细胞毒性浓度的叔丁基过氧化氢和乙醇会引发人二倍体成纤维细胞的早衰。在本研究中，我们发现，在叔丁基过氧化氢和乙醇诱导的WI-38人二倍体胎儿肺成纤维细胞早衰过程中，白细胞介素-11和血红素加氧酶-1的mRNA和蛋白质水平有所升高。我们通过基于逆转录病毒载体转导建立稳定过表达，测试白细胞介素-11和血红素加氧酶-1是否能预防叔丁基过氧化氢或乙醇诱导的早衰。结果发现，它们对增殖潜能的降低、衰老相关β-半乳糖苷酶阳性细胞比例的增加以及六个衰老相关基因mRNA水平的增加均无保护作用。
