JEG-3细胞培养上清液在混合淋巴细胞反应中会导致γ干扰素和白细胞介素-17的产生减少。
JEG-3 cell culture supernatants cause reduced interferon-gamma and interleukin-17 production in mixed-lymphocyte reactions.
作者信息
Pongcharoen Sutatip, Niumsup Pannika R, Sanguansermsri Donruedee
机构信息
Department of Medicine, Faculty of Medicine, Naresuan University, Phitsanulok 65000, Thailand.
出版信息
Am J Reprod Immunol. 2007 Mar;57(3):227-31. doi: 10.1111/j.1600-0897.2007.00467.x.
PROBLEM
Immunoregulatory effects of choriocarcinoma-derived factors on leukocytes have been documented. The present study was designed to investigate the effect of JEG-3 culture supernatants on interferon-gamma (IFN-gamma), interleukin-17 (IL-17) and IL-1beta production in the mixed lymphocyte reactions (MLRs).
METHOD OF STUDY
A human choriocarcinoma cell line JEG-3 was used to test the effects of its culture supernatants on the proliferation and cytokine production in the MLRs. The cell proliferation was assessed using the BrdU incorporation and the amounts of cytokines were measured using enzyme-linked immunosorbent assays.
RESULTS
The JEG-3 culture supernatants caused significantly reduced IFN-gamma and IL-17 production in the MLRs. However, the supernatants did not influence MLR production of IL-1beta.
CONCLUSION
IFN-gamma and IL-17 are mainly produced by activated T cells but IL-1beta is primarily produced by monocytes, thus suggesting that immunoregulatory factors of JEG-3 cells selectively inhibit cytokine production by activated T cells rather than that of the monocytes.
问题
已有文献记载绒毛膜癌衍生因子对白细胞的免疫调节作用。本研究旨在探讨JEG-3培养上清液对混合淋巴细胞反应（MLR）中γ干扰素（IFN-γ）、白细胞介素-17（IL-17）和IL-1β产生的影响。
研究方法
使用人绒毛膜癌细胞系JEG-3来检测其培养上清液对MLR中细胞增殖和细胞因子产生的影响。采用BrdU掺入法评估细胞增殖，并使用酶联免疫吸附测定法测量细胞因子的量。
结果
JEG-3培养上清液导致MLR中IFN-γ和IL-17的产生显著减少。然而，上清液不影响MLR中IL-1β的产生。
结论
IFN-γ和IL-17主要由活化的T细胞产生，而IL-1β主要由单核细胞产生，因此表明JEG-3细胞的免疫调节因子选择性抑制活化T细胞而非单核细胞产生细胞因子。
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