Naumann Bianca, Eberius Matthias, Appenroth Klaus-J
University of Jena, Institute of General Botany and Plant Physiology, Dornburger Str. 159, D-07743 Jena, Germany.
J Plant Physiol. 2007 Dec;164(12):1656-64. doi: 10.1016/j.jplph.2006.10.011. Epub 2007 Feb 12.
The duckweed Lemna minor L. clone St was used to investigate the effect of 10 heavy metals under the standardised test conditions of the ISO protocol 20079. By using growth rates derived from frond number (FN), fresh weight (FW), dry weight (DW), chlorophyll and carotenoid (Car) contents, concentration-response curves for all heavy metals and all growth parameters were classified. In addition, all data were fitted to obtain the inhibitions of growth rates (E(r)C(x)) at the level of 10%, 20% and 50% (E(r)C(10), E(r)C(20) and E(r)C(50), respectively) then used to evaluate the phytotoxicity of the different heavy metals. On the basis of the E(r)C(50) values (average ranking of all five growth parameters), the following series of phytotoxicity was detected by using molar concentrations: Ag(+)>Cd(2+)>Hg(2+)>Tl(+)>Cu(2+)>Ni(2+)>Zn(2+)>Co(2+)>Cr(VI)>As(III)>As(V).
浮萍品系Lemna minor L.克隆株St被用于在ISO协议20079的标准化测试条件下研究10种重金属的影响。通过使用从叶状体数量(FN)、鲜重(FW)、干重(DW)、叶绿素和类胡萝卜素(Car)含量得出的生长速率,对所有重金属和所有生长参数的浓度-响应曲线进行了分类。此外,对所有数据进行拟合,以获得10%、20%和50%水平下的生长速率抑制率(分别为E(r)C(10)、E(r)C(20)和E(r)C(50)),然后用于评估不同重金属的植物毒性。根据E(r)C(50)值(所有五个生长参数的平均排名),使用摩尔浓度检测到以下植物毒性系列:Ag(+)>Cd(2+)>Hg(2+)>Tl(+)>Cu(2+)>Ni(2+)>Zn(2+)>Co(2+)>Cr(VI)>As(III)>As(V)。