真核生物重组热点的染色体背景依赖性
Chromosomal context dependence of a eukaryotic recombinational hot spot.
作者信息
Ponticelli A S, Smith G R
机构信息
Fred Hutchinson Cancer Research Center, Seattle, WA 98104.
出版信息
Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):227-31. doi: 10.1073/pnas.89.1.227.
Abstract
The single base-pair mutation M26 in the ade6 gene of the fission yeast Schizosaccharomyces pombe creates a hot spot for meiotic homologous recombination. When DNA fragments containing M26 and up to 3.0 kilobases of surrounding DNA were moved to the ura4 gene or to a multicopy plasmid, M26 had no detectable hot spot activity. Our results indicate that nucleotide sequences at least 1 kilobase away from M26 are required for M26 hot spot activity and suggest that, as for transcriptional promoters, a second site or proper chromatin structure is required for activation of this eukaryotic recombinational hot spot. We discuss the implications of these results for studies of other meiotic recombinational hot spots and for gene targeting.
摘要
裂殖酵母粟酒裂殖酵母ade6基因中的单碱基对突变M26形成了减数分裂同源重组的热点。当将含有M26及多达3.0千碱基周围DNA的DNA片段转移到ura4基因或多拷贝质粒上时，M26没有可检测到的热点活性。我们的结果表明，距离M26至少1千碱基的核苷酸序列是M26热点活性所必需的，这表明，如同转录启动子一样，激活这个真核生物重组热点需要第二个位点或合适的染色质结构。我们讨论了这些结果对其他减数分裂重组热点研究和基因靶向研究的意义。
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