外周动脉疾病患者中氧化应激介导的动脉功能障碍。
Oxidative-stress-mediated arterial dysfunction in patients with peripheral arterial disease.
作者信息
Loffredo L, Marcoccia A, Pignatelli P, Andreozzi P, Borgia M C, Cangemi R, Chiarotti F, Violi Francesco
机构信息
IV Divisione di Clinica Medica, Department of Experimental Medicine and Pathology, University of Rome, La Sapienza, Roma 00161, Italy.
出版信息
Eur Heart J. 2007 Mar;28(5):608-12. doi: 10.1093/eurheartj/ehl533. Epub 2007 Feb 13.
AIMS
To investigate the existence of a relationship among flow-mediated dilation (FMD), nitric oxide (NO), and oxidative stress in patients with peripheral arterial disease (PAD), and to assess if the administration of an antioxidant was able to improve arterial dilatation.
METHODS AND RESULTS
We performed a cross-sectional study comparing FMD, 8-Hydroxy-2-deoxy-2-deoxyguanosine (8-OHdG), a marker of oxidative stress, and nitrite/nitrate (NOx) serum levels in a population of 25 PAD patients and 40 controls. In the second part of the study, 21 PAD patients were randomly allocated to a treatment sequence of 7 days of i.v. infusion of placebo or 6 g/day propionyl-L-carnitine (PLC) in a cross-over design. Compared with controls, patients with PAD had enhanced 8-OHdG serum levels (2.4 +/- 1.2 vs. 4.24 +/- 3.11 ng/mL; P < 0.001), reduced NOx (17.02 +/- 6.11 vs. 11.28 +/- 6.02 microM; P < 0.001), and lowered FMD (10.34 +/- 2.14 vs. 6.69 +/- 2.95; P < 0.001). PLC infusion was associated with an increase of FMD [from 6.6 +/- 0.6 to 11.1 +/- 1.2% (mean +/- SE), P = 0.004] and NOx (from 14.5 +/- 1.4 to 17.1 +/- 1.2 microM; +18%, P = 0.012) and a decrease of 8-OHdG (from 3.62 +/- 0.37 to 2.64 +/- 0.32 ng/mL; -27%, P < 0.001). No changes were observed after placebo treatment.
CONCLUSION
This study shows that in PAD patients, oxidative stress is implicated in determining reduced FMD.
目的
研究外周动脉疾病（PAD）患者中血流介导的血管舒张（FMD）、一氧化氮（NO）和氧化应激之间的关系，并评估给予抗氧化剂是否能够改善动脉扩张。
方法与结果
我们进行了一项横断面研究，比较了25例PAD患者和40例对照者的FMD、氧化应激标志物8-羟基-2'-脱氧鸟苷（8-OHdG）以及亚硝酸盐/硝酸盐（NOx）血清水平。在研究的第二部分，21例PAD患者采用交叉设计，被随机分配接受7天静脉输注安慰剂或6克/天丙酰-L-肉碱（PLC）的治疗方案。与对照组相比，PAD患者的8-OHdG血清水平升高（2.4±1.2对4.24±3.11纳克/毫升；P<0.001），NOx降低（17.02±6.11对11.28±6.02微摩尔；P<0.001），FMD降低（10.34±2.14对6.69±2.95；P<0.001）。输注PLC与FMD增加[从6.6±0.6%至11.1±1.2%（平均值±标准误），P = 0.004]、NOx增加（从14.5±1.4至17.1±1.2微摩尔；增加18%，P = 0.012）以及8-OHdG降低（从3.62±0.37至2.64±0.32纳克/毫升；降低27%，P<0.001）相关。安慰剂治疗后未观察到变化。
结论
本研究表明，在PAD患者中，氧化应激与FMD降低有关。
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