Wang Hai-Yan, Xu Ai-Qiang, Zhu Zhen, Li Yan, Ji Fen, Zhang Yong, Zhang Li, Xu Wen-Bo
Shandong Provincial Center for Disease Control and Prevention, Jinan 250014, China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2006 Sep;27(9):793-7.
To study the variation and evolution rules and analyze the genetic characterization of echovirus 30 during an outbreak of aseptic meningitis.
For all the echovirus 30 isolates during the outbreaks in Zhangqiu city in 2003, complete VP1 genes were determined. Phylogenetic tree reconstruction and pairwise sequence were determined to compare the homological relationship and evolution rules between Zhangqiu strains and the other reported echovirus 30 in GenBank.
Pairwise sequence comparisoms in VP1 region indicated that there were 98.9%-99.5% identity of nucleotide acid among Zhangqiu strains, and having the highest homology with Taian strains (98.5%-99.0%) isolated in Shandong province. At the same time, when comparing with Zhejiang and Jiangsu strains, the nucleotide homology showed 98.2%-98.9%, relatively low identity with Taiwan strains (87.4%-87.6%) and French strains (89.1%-89.7%) but only 82.4%-82.8% identity when comparing with echovirus 30 prototype strain Bastianni. Data from phylogenetic tree analyses indicated that all the echovirus 30 correlated with aseptic meningitis in China recently came from the same evolution linkage and formed a monophyletic cluster.
In 2003, there was an circulation of echovirus 30 in Shandong province which causing an endemic of aseptic meningitis in local areas. Data from phylogenetic tree analyses indicated that the antigenic characteristics of echovirus 30 in mainland China might be different from strains early isolated in other continents and formed a new genotype.
研究无菌性脑膜炎暴发期间肠道病毒 30 型（Echovirus 30，E30）的变异与进化规律，并分析其基因特征。
对 2003 年章丘市暴发疫情期间的所有 E30 分离株，测定其完整的 VP1 基因。通过构建系统发育树和进行成对序列比较，以分析章丘分离株与 GenBank 中其他已报道的 E30 之间的同源关系和进化规律。
VP1 区的成对序列比较显示，章丘分离株之间的核苷酸同一性为 98.9% - 99.5%，与山东省泰安市分离株的同源性最高（98.5% - 99.0%）。同时，与浙江和江苏分离株比较时，核苷酸同源性为 98.2% - 98.9%，与台湾分离株（87.4% - 87.6%）和法国分离株（89.1% - 89.7%）的同源性相对较低，而与 E30 原型株 Bastianni 的同源性仅为 82.4% - 82.8%。系统发育树分析数据表明，近期中国所有与无菌性脑膜炎相关的 E30 均来自同一进化分支，并形成一个单系簇。
2003 年山东省存在 E30 的传播，导致当地无菌性脑膜炎的流行。系统发育树分析数据表明，中国大陆 E30 的抗原特性可能与其他大陆早期分离的毒株不同，形成了一个新的基因型。
