Key Laboratory for Pediatrics of Integrated Traditional and Western Medicine, Liaocheng People's Hospital, No. 67 Dongchang West Road, Liaocheng, 252000, Shandong Province, PR China.
BMC Infect Dis. 2021 May 1;21(1):405. doi: 10.1186/s12879-021-06112-9.
Aseptic meningitis is most often caused by enteroviruses (EVs), but EVs associated with aseptic meningitis have not yet been reported in Liaocheng. The aim of this study was to determine the prevalence and genetic characteristics of EVs causing aseptic meningitis in children in Liaocheng.
We reviewed the epidemiological and clinical characteristics of 504 paediatric cases of aseptic meningitis in Liaocheng from 2018 to 2019 and analysed the phylogeny of the predominant EV types causing this disease.
A total of 107 children were positive for EV in cerebrospinal fluid samples by nested PCR. Most of the positive patients were children 13 years old or younger and had symptoms such as fever, headache and vomiting (P < 0.05). The seasons with the highest prevalence of EV-positive cases were summer and autumn. The 107 EV sequences belonged to 8 serotypes, and echovirus types 18, 6 and 11 were the three dominant serotypes in Liaocheng during the 2-year study period. Phylogenetic analyses demonstrated that the E18 and E6 isolates belonged to subgenotype C2, while the E11 isolates belonged to subgenotype D5. VP1 analysis suggested that only one lineage of these three types was cocirculating in the Liaocheng region.
This study demonstrated the diverse EV genotypes contributing to a large outbreak of aseptic meningitis in Liaocheng. Therefore, large-scale surveillance is required to assess the epidemiology of EVs associated with aseptic meningitis and is important for the diagnosis and treatment of aseptic meningitis in Liaocheng.
无菌性脑膜炎最常由肠病毒（EV）引起，但在聊城尚未报告与无菌性脑膜炎相关的 EV。本研究旨在确定引起聊城儿童无菌性脑膜炎的 EV 的流行率和遗传特征。
我们回顾了 2018 年至 2019 年聊城 504 例儿童无菌性脑膜炎的流行病学和临床特征，并分析了引起该病的主要 EV 型的系统发育。
通过巢式 PCR，共有 107 例脑脊液样本中 EV 呈阳性。大多数阳性患者为 13 岁或以下的儿童，有发热、头痛和呕吐等症状（P<0.05）。EV 阳性病例高发的季节是夏季和秋季。107 个 EV 序列属于 8 个血清型，在 2 年的研究期间，肠病毒 18、6 和 11 是聊城的三种主要血清型。系统发育分析表明，E18 和 E6 分离株属于 C2 亚组，而 E11 分离株属于 D5 亚组。VP1 分析表明，这三种类型只有一个谱系在聊城地区共同流行。
本研究表明，不同的 EV 基因型导致了聊城大规模无菌性脑膜炎的爆发。因此，需要进行大规模监测，以评估与无菌性脑膜炎相关的 EV 的流行病学情况，这对于聊城无菌性脑膜炎的诊断和治疗非常重要。
