Tao Zexin, Wang Haiyan, Li Yan, Liu Guifang, Xu Aiqiang, Lin Xiaojuan, Song Lizhi, Ji Feng, Wang Suting, Cui Ning, Song Yanyan
Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China ; Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China.
Academy of Preventive Medicine, Shandong University, Jinan, People's Republic of China ; Shandong Provincial Key Laboratory of Infectious Disease Control and Prevention, Shandong Center for Disease Control and Prevention, Jinan, People's Republic of China ; School of Public Health, Shandong University, Jinan, People's Republic of China.
PLoS One. 2014 Feb 21;9(2):e89766. doi: 10.1371/journal.pone.0089766. eCollection 2014.
Human enteroviruses (HEVs) are common causes of acute meningitis. However, there is limited information about HEV associated with aseptic meningitis in mainland China because it has not been classified as a notifiable disease.
To characterize the HEVs associated with sporadic aseptic meningitis in China and to analyze their genetic features.
Cerebrospinal fluid, throat swab and feces specimens were collected from patients with aseptic meningitis in 5 sentinel hospitals in Shandong Province, China between 2006 and 2012. Virological investigation (viral isolation and molecular identification) and phylogenetic analysis were performed.
A total of 437 hospitalized patients were reported, and enteroviruses were detected in the specimens from 84 patients (19.2%) and were identified into 17 serotypes. The nine main serotypes were echovirus (E) 30 (27.4%), EV71 (13.1%), coxsackievirus (CV) B1 (9.5%), CVB3 (7.1%), CVB5 (7.1%), E6 (7.1%), E9 (7.1%), CVA9 (6.0%), and CVA10 (3.6%). Monthly distribution of isolated enteroviruses revealed a major peak in summer-fall season and a small second peak in winter constituted totally by EV71. Sequence analysis on VP1 coding region suggested Shandong strains had great genetic divergence with isolates from other countries.
Multiple serotypes were responsible for enterovirus meningitis in mainland China. Aseptic meningitis caused by EV71 and coxsackie A viruses-the predominant pathogens for the hand, foot, and mouth disease-is currently an important concern in mainland China.
人肠道病毒（HEVs）是急性脑膜炎的常见病因。然而，在中国内地，由于未将其列为法定报告疾病，关于与无菌性脑膜炎相关的HEV的信息有限。
对中国散发性无菌性脑膜炎相关的HEVs进行特征分析，并分析其基因特征。
2006年至2012年期间，从中国山东省5家哨点医院的无菌性脑膜炎患者中采集脑脊液、咽拭子和粪便标本。进行病毒学调查（病毒分离和分子鉴定）及系统发育分析。
共报告437例住院患者，84例患者（19.2%）的标本中检测到肠道病毒，并鉴定出17种血清型。9种主要血清型为埃可病毒（E）30型（27.4%）、肠道病毒71型（EV71，13.1%）、柯萨奇病毒（CV）B1型（9.5%）、CVB3型（7.1%）、CVB5型（7.1%）、E6型（7.1%）、E9型（7.1%）、柯萨奇病毒A9型（CVA9，6.0%）和柯萨奇病毒A10型（CVA10，3.6%）。分离出的肠道病毒的月度分布显示，夏秋季有一个主要高峰，冬季有一个由EV71构成的小的第二个高峰。VP1编码区的序列分析表明，山东毒株与其他国家的分离株有很大的基因差异。
多种血清型导致中国内地的肠道病毒脑膜炎。由EV71和柯萨奇A病毒（手足口病的主要病原体）引起的无菌性脑膜炎是中国内地目前的一个重要关注点。
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