Monecke Stefan, Kuhnert Peter, Hotzel Helmut, Slickers Peter, Ehricht Ralf
Institute for Medical Microbiology and Hygiene, Faculty of Medicine, Carl Gustav Carus at the Technical University of Dresden, Fetscherstrasse 74, D-01307, Dresden, Germany.
Vet Microbiol. 2007 Nov 15;125(1-2):128-40. doi: 10.1016/j.vetmic.2007.05.016. Epub 2007 May 24.
Staphylococcus aureus is a common pathogen which can colonise and infect not only man, but also domestic animals. Especially, infection of cattle is of high economic relevance as S. aureus is an important causal agent of bovine mastitis. In the present contribution, a DNA microarray was applied for the study of 144 different gene targets, including resistance genes and genes encoding exotoxins, in S. aureus isolated from cows. One hundred and twenty-eight isolates from Germany and Switzerland were tested. These isolates were assigned to 20 different strains and nine clonal complexes. The majority of isolates belonged either to apparently closely related clonal complexes 8, 25, and 97 (together 34.4%) or were related to the sequenced bovine strain RF122 (48.4%). Notable characteristics of S. aureus of bovine origin are the carriage of intact haemolysin beta (in 82% of isolates tested), the absence of staphylokinase (in 89.1%), the presence of allelic variants of several exotoxins such as toxic shock syndrome toxin and enterotoxin N, and the occurrence of the leukocidin lukF-P83/lukM (in 53.1%). Two isolates were methicillin-resistant S. aureus (MRSA). One of them was a clonal complex 8 MRSA related to the epidemic MRSA strain Irish 01. The other one belonged to ST398/spa-type 34 resembling a newly emerging MRSA strain which has been described to occur in humans as well as in domestic animals. The presence of these two strains highlights the possibility of transfers of S. aureus strains between different host species.
金黄色葡萄球菌是一种常见病原体，它不仅可在人类中定植和感染，还能在家畜中出现。特别是，牛感染金黄色葡萄球菌具有很高的经济相关性，因为它是牛乳腺炎的重要致病因子。在本研究中，利用DNA微阵列对从奶牛分离出的金黄色葡萄球菌的144个不同基因靶点进行了研究，这些靶点包括耐药基因和编码外毒素的基因。对来自德国和瑞士的128株分离株进行了检测。这些分离株被归为20个不同菌株和9个克隆复合体。大多数分离株要么属于明显密切相关的克隆复合体8、25和97（共占34.4%），要么与已测序的牛菌株RF122相关（占48.4%）。牛源金黄色葡萄球菌的显著特征包括携带完整的β溶血素（在所检测的分离株中占82%）、缺乏葡萄球菌激酶（占89.1%）、存在几种外毒素的等位基因变体，如中毒性休克综合征毒素和肠毒素N，以及白细胞毒素lukF-P83/lukM的出现（占53.1%）。有两株分离株是耐甲氧西林金黄色葡萄球菌（MRSA）。其中一株是与流行的MRSA菌株爱尔兰01相关的克隆复合体8 MRSA。另一株属于ST398/spa型34，类似于一种新出现的MRSA菌株，该菌株已被描述在人类和家畜中均有出现。这两种菌株的存在凸显了金黄色葡萄球菌菌株在不同宿主物种间转移的可能性。
