5号染色体的缺失和片段化是与白色念珠菌rad52-ΔΔ菌株适应山梨糖相关的主要事件。
Loss and fragmentation of chromosome 5 are major events linked to the adaptation of rad52-DeltaDelta strains of Candida albicans to sorbose.
作者信息
Andaluz Encarnación, Gómez-Raja Jonathan, Hermosa Belén, Ciudad Toni, Rustchenko E, Calderone Richard, Larriba Germán
机构信息
Departamento de Microbiología, Facultad de Ciencias, Universidad de Extremadura, 06071 Badajoz, Spain.
出版信息
Fungal Genet Biol. 2007 Aug;44(8):789-98. doi: 10.1016/j.fgb.2007.01.005. Epub 2007 Jan 11.
Candida albicans can adapt and grow on sorbose plates by losing one copy of Chr5. Since rad52 mutants of Saccharomyces cerevisiae lose chromosomes at a high rate, we have investigated the ability of C. albicans rad52 to adapt to sorbose. Carad52-DeltaDelta mutants generate Sou(+) strains earlier than wild-type but the final yield is lower, probably because they die at a higher rate in sorbose. As other strains of C. albicans, CAF2 and rad52-DeltaDelta derivatives generate Sou(+) strains by a loss of one copy of Chr5 about 75% of the time. In addition, rad52 strains were able to produce Sou(+) strains by a fragmentation/deletion event in one copy of Chr5, consisting of loss of a region adjacent to the right telomere. Finally, both CAF2 and rad52-DeltaDelta produced Sou(+) strains with two apparent full copies of Chr5, suggesting that additional genomic changes may also regulate adaptation to sorbose.
白色念珠菌可以通过丢失一条5号染色体拷贝来适应并在山梨糖平板上生长。由于酿酒酵母的rad52突变体以高频率丢失染色体，我们研究了白色念珠菌rad52适应山梨糖的能力。白色念珠菌rad52-ΔΔ突变体比野生型更早产生山梨糖抗性（Sou(+)）菌株，但最终产量较低，可能是因为它们在山梨糖中死亡率更高。与其他白色念珠菌菌株一样，CAF2和rad52-ΔΔ衍生物约75%的情况下通过丢失一条5号染色体拷贝产生Sou(+)菌株。此外，rad52菌株能够通过5号染色体一个拷贝中的片段化/缺失事件产生Sou(+)菌株，该事件包括右端粒附近区域的丢失。最后，CAF2和rad52-ΔΔ都产生了具有两条明显完整5号染色体拷贝的Sou(+)菌株，这表明其他基因组变化也可能调节对山梨糖的适应性。
Zotero 插件